1 Ocak 2020 Dergi makalesi Açık Erişim

Kahremanoglu, Kubra; Temel, Ezgi Rana; Korkut, Tamara Ecem; Nalbant, Atakan Arda; Azer, Bersu Bastug; Durucan, Caner; Volkan, Murvet; Boyaci, Ezel

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        "affiliation": "Middle East Tech Univ, Dept Chem, TR-06800 Ankara, Turkey", 
        "name": "Kahremanoglu, Kubra"
      }, 
      {
        "affiliation": "Middle East Tech Univ, Dept Chem, TR-06800 Ankara, Turkey", 
        "name": "Temel, Ezgi Rana"
      }, 
      {
        "affiliation": "Middle East Tech Univ, Dept Chem, TR-06800 Ankara, Turkey", 
        "name": "Korkut, Tamara Ecem"
      }, 
      {
        "affiliation": "Middle East Tech Univ, Dept Chem, TR-06800 Ankara, Turkey", 
        "name": "Nalbant, Atakan Arda"
      }, 
      {
        "name": "Azer, Bersu Bastug"
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      {
        "name": "Durucan, Caner"
      }, 
      {
        "affiliation": "Middle East Tech Univ, Dept Chem, TR-06800 Ankara, Turkey", 
        "name": "Volkan, Murvet"
      }, 
      {
        "affiliation": "Middle East Tech Univ, Dept Chem, TR-06800 Ankara, Turkey", 
        "name": "Boyaci, Ezel"
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    "description": "Recently the connection between oxidative stress and various diseases, including cancer and Alzheimer's, attracts notice as a pathway suitable for diagnostic purposes. 8-Oxo-deoxyguanosine and 8-oxo-deoxyadenosine produced from the interaction of reactive oxygen species with DNA become prominent as biomarkers. Several methods have been developed for their determination in biofluids, including solid-phase extraction and enzyme-linked immunosorbent assays. However, still, there is a need for reliable and fast analytical methods. In this context, solid-phase microextraction offers many advantages such as flexibility in geometry and applicable sample volume, as well as high adaptability to high-throughput sampling. In this study, a solid-phase microextraction method was developed for the determination of 8-oxo-deoxyguanosine and 8-oxo-deoxyadenosine in biofluids. The extractive phase of solid-phase microextraction consisted of hydrophilic-lipophilic balanced polymeric particles. In order to develop a solid-phase microextraction method suitable for the determination of the analytes in saliva and urine, several parameters, including desorption solvent, desorption time, sample pH, and ionic strength, were scrutinized. Analytical figures of merit indicated that the developed method provides reasonable interday and intraday precisions (<15% in both biofluids) with acceptable accuracy. The method provides a limit of quantification for both biomarkers at 5.0 and 10.0 ng/mL levels in saliva and urine matrices, respectively.", 
    "doi": "10.1002/jssc.202000211", 
    "has_grant": false, 
    "journal": {
      "issue": "9-10", 
      "pages": "1925-1933", 
      "title": "JOURNAL OF SEPARATION SCIENCE", 
      "volume": "43"
    }, 
    "license": {
      "id": "cc-by"
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    "publication_date": "2020-01-01", 
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    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
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    "title": "Development of a solid-phase microextraction LC-MS/MS method for determination of oxidative stress biomarkers in biofluids"
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