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The utility of NBS-profiling for characterization of yellow rust resistance in an F-6 durum wheat population

Tufan, Hale A.; Taskin, Belgin Gocmen; Maccormack, Ruth; Boyd, Lesley A.; Kaya, Zeki; Turet, Muge


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        "affiliation": "Bogazici Univ, Dept Mol Biol & Genet, TR-34342 Istanbul, Turkey", 
        "name": "Tufan, Hale A."
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      {
        "affiliation": "Mugla Sitki Kocman Univ, Dept Biol, TR-48100 Mugla, Turkey", 
        "name": "Taskin, Belgin Gocmen"
      }, 
      {
        "affiliation": "John Innes Ctr, Dept Dis & Stress Biol, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England", 
        "name": "Maccormack, Ruth"
      }, 
      {
        "name": "Boyd, Lesley A."
      }, 
      {
        "affiliation": "Middle East Tech Univ, Dept Biol, TR-06531 Ankara, Turkey", 
        "name": "Kaya, Zeki"
      }, 
      {
        "affiliation": "Bogazici Univ, Dept Mol Biol & Genet, TR-34342 Istanbul, Turkey", 
        "name": "Turet, Muge"
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    "description": "Seedling and adult plant (field) resistance to yellow rust in the durum wheat (Triticum turgidum ssp. durum) cross Kunduru-1149 x Cham-1 was characterized using a functionally-targeted DNA marker system, NBS-profiling. Chi-squared analysis indicated a four gene model conferring seedling yellow rust resistance against Puccinia striiformis f. sp. tritici isolate WYR85/22 (virulent on Yr2, Yr6, Yr7 and Yr9). Interval mapping located two QTL for yellow rust resistance on the long arm of chromosome 1B, while Kruskal-Wallis single marker regression identified a number of additional marker loci associated with seedling and/or adult plant, field resistance to yellow rust. These results suggested that much of the yellow rust resistance seen in the field may be due to seedling expressed resistance (R) genes. Characterization of the DNA sequence of three NBS marker loci indicated that all showed significant homology to functionally-characterized R-genes and resistance gene analogues (RGAs), with the greatest homology being NBS-LRR-type R-genes and RGAs from cereal species.", 
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    "title": "The utility of NBS-profiling for characterization of yellow rust resistance in an F-6 durum wheat population"
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