1 Ocak 2019 Dergi makalesi Açık Erişim

Taskin, Ali Cihan; Kocabay, Ahmet; Ebrahimi, Ayyub; Karahuseyinoglu, Sercin; Sahin, Gizem Nur; Ozcimen, Burcu; Ruacan, Arzu; Onder, Tamer T.

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    "creators": [
      {
        "affiliation": "Koc Univ, Res Ctr Translat Med, Embryo Manipulat Lab, Anim Res Facil, TR-34450 Istanbul, Turkey", 
        "name": "Taskin, Ali Cihan"
      }, 
      {
        "affiliation": "Koc Univ, Res Ctr Translat Med, Embryo Manipulat Lab, Anim Res Facil, TR-34450 Istanbul, Turkey", 
        "name": "Kocabay, Ahmet"
      }, 
      {
        "affiliation": "Halic Univ, Fac Arts & Sci, Mol Biol & Genet Dept, Sutluce Mah,Imrahor Cad 82, TR-34445 Istanbul, Turkey", 
        "name": "Ebrahimi, Ayyub"
      }, 
      {
        "affiliation": "Koc Univ, Sch Med, Dept Histol & Embryol, TR-34450 Istanbul, Turkey", 
        "name": "Karahuseyinoglu, Sercin"
      }, 
      {
        "affiliation": "Koc Univ, Sch Med, Dept Histol & Embryol, TR-34450 Istanbul, Turkey", 
        "name": "Sahin, Gizem Nur"
      }, 
      {
        "affiliation": "Koc Univ, Sch Med, Dept Mol Biol & Genet, TR-34450 Istanbul, Turkey", 
        "name": "Ozcimen, Burcu"
      }, 
      {
        "affiliation": "Koc Univ, Sch Med, Dept Pathol, TR-34450 Istanbul, Turkey", 
        "name": "Ruacan, Arzu"
      }, 
      {
        "affiliation": "Koc Univ, Sch Med, Dept Mol Biol & Genet, TR-34450 Istanbul, Turkey", 
        "name": "Onder, Tamer T."
      }
    ], 
    "description": "Leptin, a metabolic hormone, regulates the reproductive functions responding to both nutritional and body conditions. Embryonic stem cells play important roles in reproductive technology, but their derivation can be challenging. In this study, we evaluated the derivation rates of mouse embryonic stem cell (mESC) line from blastocysts developing in embryo culture media supplemented with different leptin concentrations. The results showed that addition of leptin into the embryo culture medium supported the in vitro development of mouse embryo. The mESC line derivation rates for media treated with 0, 10, 50, and 100ng/ml of leptin were 61.24 % (54/88), 84.96 % (42/50), 81.79 % (61/76), and 85.78 % (56/67), respectively. In addition, leptin treatment of blastocysts upregulated the expression levels of the trophectoderm marker Cdx2, whereas inner cell mass markers Oct-4 and Nanog were not affected. mESC lines derived after leptin treatment demonstrated hallmarks of pluripotency, such as alkaline phosphatase activity, expression of, OCT4, NANOG, and SSEA1, as well as the ability to form embryoid bodies and well-differentiated teratomas. In conclusion, leptin has a positive effect on the derivation rate of mouse embryonic stem cell lines which may be, in part, due to its effects on the development of the trophectoderm cell lineage in the embryo.", 
    "doi": "10.1007/s11626-019-00367-y", 
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    "journal": {
      "issue": "7", 
      "pages": "473-481", 
      "title": "IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL", 
      "volume": "55"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2019-01-01", 
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    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
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    "title": "Leptin treatment of in vitro cultured embryos increases outgrowth rate of inner cell mass during embryonic stem cell derivation"
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