1 Ocak 2019 Dergi makalesi Açık Erişim

Alan, Saadet; Salva, Emine; Yilmaz, Ismet; Turan, Suna Ozbas; Akbuga, Julide

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    "creators": [
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        "affiliation": "Inonu Univ, Fac Med, Dept Med Pathol, Malatya, Turkey", 
        "name": "Alan, Saadet"
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      {
        "affiliation": "Inonu Univ, Fac Pharm, Dept Pharmaceut Biotechnol, Malatya, Turkey", 
        "name": "Salva, Emine"
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      {
        "affiliation": "Inonu Univ, Fac Pharm, Dept Pharmacol, Malatya, Turkey", 
        "name": "Yilmaz, Ismet"
      }, 
      {
        "affiliation": "Marmara Univ, Fac Pharm, Dept Pharmaceut Biotechnol, Istanbul, Turkey", 
        "name": "Turan, Suna Ozbas"
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      {
        "affiliation": "Marmara Univ, Fac Pharm, Dept Pharmaceut Biotechnol, Istanbul, Turkey", 
        "name": "Akbuga, Julide"
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    "description": "Platelet-derived growth factor-B (PDGF-B) is a growth factor that plays an important role in the progression of mesangial proliferative glomerulonephritis (MsPGN). PDGF-B may contribute to mesangioproliferative changes and is overexpressed in MsPGN. Recently, small interfering RNAs (siRNAs) have been widely used for gene silencing effects in experimental models of renal diseases. Nanoparticle-based therapeutics are preferred for reasons such as increasing therapeutic efficacy and reducing toxic effects caused by high doses. The distribution of nanoparticles to the kidney is a significant advantage in siRNA delivery. The aim of this study was to investigate the efficacy of chitosan/siRNA nanoplexes in silencing of PDGF-B and PDGFR-beta genes in kidney and to decrease mesangial cell proliferation and matrix accumulation in MsPGN model induced by anti-Thy-1.1 antibody. The therapeutic effects of chitosan/siPDGF-B + siPDGFR-beta nanoplexes in glomerulonephritic rats were studied by molecular, biochemical, and histopathologic evaluations. Chitosan/siPDGF-B + siPDGFR-beta nanoplexes markedly reduced PDGF-B and PDGFR-beta mRNA and protein expressions in experimental MsPGN model. Histopathologic examination results showed that the silencing of PDGF-B and its receptor PDGFR-beta led to reduction in mesangial cell proliferation and matrix accumulation. The use of chitosan/siPDGF-B + siPDGFR-beta nanoplexes for silencing the PDGF-B pathway in MsPGN can be considered as a new effective therapeutic strategy.", 
    "doi": "10.1016/j.yexmp.2019.104280", 
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    "journal": {
      "title": "EXPERIMENTAL AND MOLECULAR PATHOLOGY", 
      "volume": "110"
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    "publication_date": "2019-01-01", 
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      "title": "Dergi makalesi", 
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    "title": "The effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-beta in the mesangial proliferative glomerulonephritis therapy"
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