1 Ocak 2019 Dergi makalesi Açık Erişim

Bode, Edna; Heinrich, Antje K.; Hirschmann, Merle; Abebew, Desalegne; Shi, Yan-Ni; Tien Duy Vo; Wesche, Frank; Shi, Yi-Ming; Gruen, Peter; Simonyi, Svenja; Keller, Nadine; Engel, Yvonne; Wenski, Sebastian; Bennet, Reuel; Beyer, Sophie; Bischoff, Iris; Buaya, Anthony; Brandt, Sophie; Cakmak, Ibrahim; Cimen, Harun; Cimen, Harun

JSON-LD (schema.org)

{
  "@context": "https://schema.org/", 
  "@id": 67757, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Bode, Edna"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Heinrich, Antje K."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Hirschmann, Merle"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Abebew, Desalegne"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Shi, Yan-Ni"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Tien Duy Vo"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Wesche, Frank"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Shi, Yi-Ming"
    }, 
    {
      "@type": "Person", 
      "name": "Gruen, Peter"
    }, 
    {
      "@type": "Person", 
      "name": "Simonyi, Svenja"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Keller, Nadine"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Engel, Yvonne"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Mol Biotechnol, D-60438 Frankfurt, Germany", 
      "name": "Wenski, Sebastian"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Senckenberg BiK F, D-60325 Frankfurt, Germany", 
      "name": "Bennet, Reuel"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Dept Dev Biol Vertebrates, Cell Biol & Neurosci, D-60438 Frankfurt, Germany", 
      "name": "Beyer, Sophie"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Goethe Univ Frankfurt, Pharmaceut Biol, Fac Biochem Chem & Pharm, D-60438 Frankfurt, Germany", 
      "name": "Bischoff, Iris"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Senckenberg BiK F, D-60325 Frankfurt, Germany", 
      "name": "Buaya, Anthony"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Hamburg, Mol Phytopathol, D-22609 Hamburg, Germany", 
      "name": "Brandt, Sophie"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Adnan Menderes Univ, Dept Plant Protect, TR-09100 Aydin, Turkey", 
      "name": "Cakmak, Ibrahim"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Adnan Menderes Univ, Dept Biol, TR-09100 Aydin, Turkey", 
      "name": "Cimen, Harun"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Adnan Menderes Univ, Dept Biol, TR-09100 Aydin, Turkey", 
      "name": "Cimen, Harun"
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene-sequencesimilarity-based bioinformatics strategies, the actual access to these NPs for structure elucidation and bioactivity testing remains difficult. Deletion of the gene encoding the RNA chaperone, Hfq, results in strains losing the production of most NPs. By exchanging the native promoter of a desired BGC against an inducible promoter in Dhfq mutants, almost exclusive production of the corresponding NP from the targeted BGC in Photorhabdus, Xenorhabdus and Pseudomonas was observed including the production of several new NPs derived from previously uncharacterized non-ribosomal peptide synthetases (NRPS). This easyPACId approach (easy Promoter Activated Compound Identification) facilitates NP identification due to low interference from other NPs. Moreover, it allows direct bioactivity testing of supernatants containing secreted NPs, without laborious purification.", 
  "headline": "Promoter Activation in Delta hfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing", 
  "identifier": 67757, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Promoter Activation in Delta hfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing", 
  "url": "https://aperta.ulakbim.gov.tr/record/67757"
}