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Irfan, M.; Guler, H. I.; Belduz, A. O.; Shah, A. A.; Canakci, S.
<?xml version='1.0' encoding='UTF-8'?> <record xmlns="http://www.loc.gov/MARC21/slim"> <leader>00000nam##2200000uu#4500</leader> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Guler, H. I.</subfield> <subfield code="u">Karadeniz Tech Univ, Dept Departmet Mol Biol & Genet, Fac Sci, TR-61080 Trabzon, Turkey</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Belduz, A. O.</subfield> <subfield code="u">Karadeniz Tech Univ, Dept Biol, Fac Sci, TR-61080 Trabzon, Turkey</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Shah, A. A.</subfield> <subfield code="u">Quaid I Azam Univ, Dept Microbiol, Fac Biol Sci, Islamabad, Pakistan</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Canakci, S.</subfield> <subfield code="u">Karadeniz Tech Univ, Dept Biol, Fac Sci, TR-61080 Trabzon, Turkey</subfield> </datafield> <datafield tag="909" ind1="C" ind2="4"> <subfield code="p">APPLIED BIOCHEMISTRY AND MICROBIOLOGY</subfield> <subfield code="v">52</subfield> <subfield code="n">3</subfield> <subfield code="c">277-286</subfield> </datafield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="540" ind1=" " ind2=" "> <subfield code="a">Creative Commons Attribution</subfield> <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield> </datafield> <datafield tag="024" ind1=" " ind2=" "> <subfield code="a">10.1134/S0003683816030066</subfield> <subfield code="2">doi</subfield> </datafield> <datafield tag="245" ind1=" " ind2=" "> <subfield code="a">Cloning, purification and characterization of a cellulase-free xylanase from Geobacillus thermodenitrificans AK53</subfield> </datafield> <datafield tag="100" ind1=" " ind2=" "> <subfield code="a">Irfan, M.</subfield> <subfield code="u">Quaid I Azam Univ, Dept Microbiol, Fac Biol Sci, Islamabad, Pakistan</subfield> </datafield> <datafield tag="909" ind1="C" ind2="O"> <subfield code="o">oai:zenodo.org:60711</subfield> <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="650" ind1="1" ind2="7"> <subfield code="2">opendefinition.org</subfield> <subfield code="a">cc-by</subfield> </datafield> <datafield tag="260" ind1=" " ind2=" "> <subfield code="c">2016-01-01</subfield> </datafield> <datafield tag="856" ind1="4" ind2=" "> <subfield code="u">https://aperta.ulakbim.gov.trrecord/60711/files/bib-119efbbc-2d6b-4efa-8f1e-e8ad6aea207f.txt</subfield> <subfield code="z">md5:675a375d62b11d7013574bafbf263bb6</subfield> <subfield code="s">234</subfield> </datafield> <datafield tag="542" ind1=" " ind2=" "> <subfield code="l">open</subfield> </datafield> <controlfield tag="005">20210316010332.0</controlfield> <controlfield tag="001">60711</controlfield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">publication</subfield> <subfield code="b">article</subfield> </datafield> <datafield tag="520" ind1=" " ind2=" "> <subfield code="a">Geobacillus thermodenitrificans AK53 xyl gene encoding xylanase was isolated, cloned and expressed in Escherichia coli. After purifying recombinant xylanase from G. thermodenitrificans AK53 (GthAK53Xyl) to homogeneity by ammonium sulfate precipitation and ion exchange chromatography, biochemical properties of the enzyme were determined. The kinetic studies for GthAK53Xyl showed K-M value to be 4.34 mg/mL (for D-xylose) and V-max value to be 2028.9 mu moles mg(-1) min(-1). The optimal temperature and pH for enzyme activity were found out to be 70 degrees C and 5.0, respectively. The expressed protein showed the highest sequence similarity with the xylanases of G. thermodenitrificans JK1 (JN209933) and G. thermodenitrificans T-2 (EU599644). Metal cations Mg2+ and Mn2+ were found to be required for the enzyme activity, however, Co2+, Hg2+, Fe2+ and Cu2+ ions caused inhibitor effect on it. GthAK53Xyl had no cellulolytic activity and degraded xylan in an endo-fashion. The action of the enzyme on xylan from oat spelt produced xylobiose and xylopentose. The reported results are suggestive of a xylanase exhibiting desirable kinetics, stability parameters and metal resistance required for the efficient production of xylobiose at industrial scale.</subfield> </datafield> </record>
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