Dergi makalesi Açık Erişim
Ozketen, Ahmet Caglar; Andac-Ozketen, Ayse; Dagvadorj, Bayantes; Demiralay, Burak; Akkaya, Mahinur S.
<?xml version='1.0' encoding='UTF-8'?> <record xmlns="http://www.loc.gov/MARC21/slim"> <leader>00000nam##2200000uu#4500</leader> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Andac-Ozketen, Ayse</subfield> <subfield code="u">Middle East Tech Univ, Biotechnol Program, TR-06800 Ankara, Turkey</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Dagvadorj, Bayantes</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Demiralay, Burak</subfield> <subfield code="u">Middle East Tech Univ, Biotechnol Program, TR-06800 Ankara, Turkey</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Akkaya, Mahinur S.</subfield> <subfield code="u">Dalian Univ Technol, Sch Bioengn, 2 Linggong Rd, Dalian 116023, Liaoning, Peoples R China</subfield> </datafield> <datafield tag="909" ind1="C" ind2="4"> <subfield code="p">BIOSCIENCE REPORTS</subfield> <subfield code="v">40</subfield> </datafield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="540" ind1=" " ind2=" "> <subfield code="a">Creative Commons Attribution</subfield> <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield> </datafield> <datafield tag="024" ind1=" " ind2=" "> <subfield code="a">10.1042/BSR20201188</subfield> <subfield code="2">doi</subfield> </datafield> <datafield tag="245" ind1=" " ind2=" "> <subfield code="a">In-depth secretome analysis of Puccinia striiformis f. sp. tritici in infected wheat uncovers effector functions</subfield> </datafield> <datafield tag="100" ind1=" " ind2=" "> <subfield code="a">Ozketen, Ahmet Caglar</subfield> <subfield code="u">Middle East Tech Univ, Biotechnol Program, TR-06800 Ankara, Turkey</subfield> </datafield> <datafield tag="909" ind1="C" ind2="O"> <subfield code="o">oai:zenodo.org:5997</subfield> <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="650" ind1="1" ind2="7"> <subfield code="2">opendefinition.org</subfield> <subfield code="a">cc-by</subfield> </datafield> <datafield tag="260" ind1=" " ind2=" "> <subfield code="c">2020-01-01</subfield> </datafield> <datafield tag="856" ind1="4" ind2=" "> <subfield code="u">https://aperta.ulakbim.gov.trrecord/5997/files/bib-1648a227-94e3-4f7b-9fb7-76ee26743c9f.txt</subfield> <subfield code="z">md5:badc74d8e08e53c5af76e21a960e4946</subfield> <subfield code="s">225</subfield> </datafield> <datafield tag="542" ind1=" " ind2=" "> <subfield code="l">open</subfield> </datafield> <controlfield tag="005">20210315061924.0</controlfield> <controlfield tag="001">5997</controlfield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">publication</subfield> <subfield code="b">article</subfield> </datafield> <datafield tag="520" ind1=" " ind2=" "> <subfield code="a">The importance of wheat yellow rust disease, caused by Puccinia striiformis f. sp. tritici (Pst), has increased substantially due to the emergence of aggressive new Pst races in the last couple of decades. In an era of escalating human populations and climate change, it is vital to understand the infection mechanism of Pst in order to develop better strategies to combat wheat yellow disease. The present study focuses on the identification of small secreted proteins (SSPs) and candidate-secreted effector proteins (CSEPs) that are used by the pathogen to support infection and control disease development. We generated de novo assembled transcriptomes of Pst collected from wheat fields in central Anatolia. We inoculated both susceptible and resistant seedlings with Pst and analyzed haustoria formation. At 10 days post-inoculation (dpi), we analyzed the transcriptomes and identified 10550 Differentially Expressed Unigenes (DEGs), of which 6072 were Pst-mapped. Among those Pst-related genes, 227 were predicted as PstSSPs. In silico characterization was performed using an approach combining the transcriptomic data and datamining results to provide a reliable list to narrow down the ever-expanding repertoire of predicted effectorome. The comprehensive analysis detected 14 Differentially Expressed Small-Secreted Proteins (DESSPs) that overlapped with the genes in available literature data to serve as the best CSEPs for experimental validation. One of the CSEPs was cloned and studied to test the reliability of the presented data. Biological assays show that the randomly selected CSEP, Unigene17495 (PSTG 10917), localizes in the chloroplast and is able to suppress cell death induced by INF1 in a Nicotiana benthamiana heterologous expression system.</subfield> </datafield> </record>
Görüntülenme | 20 |
İndirme | 2 |
Veri hacmi | 450 Bytes |
Tekil görüntülenme | 18 |
Tekil indirme | 2 |