1 Ocak 2020 Dergi makalesi Açık Erişim

Keskin, Nazan; Erdogan, Cennet; Bucak, Mustafa Numan; Ozturk, Ali Erdem; Bodu, Mustafa; Ili, Pinar; Baspinar, Nuri; Dursun, Sukru

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/5717</identifier>
  <creators>
    <creator>
      <creatorName>Keskin, Nazan</creatorName>
      <givenName>Nazan</givenName>
      <familyName>Keskin</familyName>
      <affiliation>Pamukkale Univ, Dept Histol &amp; Embryol, Fac Med, Denizli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Erdogan, Cennet</creatorName>
      <givenName>Cennet</givenName>
      <familyName>Erdogan</familyName>
      <affiliation>Pamukkale Univ, Dept Histol &amp; Embryol, Grad Sch Hlth Sci, TR-20070 Denizli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Bucak, Mustafa Numan</creatorName>
      <givenName>Mustafa Numan</givenName>
      <familyName>Bucak</familyName>
      <affiliation>Selcuk Univ, Vet Fac, Dept Reprod &amp; Artificial Inseminat, TR-42003 Konya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ozturk, Ali Erdem</creatorName>
      <givenName>Ali Erdem</givenName>
      <familyName>Ozturk</familyName>
      <affiliation>Selcuk Univ, Vet Fac, Dept Reprod &amp; Artificial Inseminat, TR-42003 Konya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Bodu, Mustafa</creatorName>
      <givenName>Mustafa</givenName>
      <familyName>Bodu</familyName>
      <affiliation>Selcuk Univ, Vet Fac, Dept Reprod &amp; Artificial Inseminat, TR-42003 Konya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ili, Pinar</creatorName>
      <givenName>Pinar</givenName>
      <familyName>Ili</familyName>
      <affiliation>Pamukkale Univ, Denizli Hlth Serv, Vocat High Sch, Denizli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Baspinar, Nuri</creatorName>
      <givenName>Nuri</givenName>
      <familyName>Baspinar</familyName>
      <affiliation>Selcuk Univ, Vet Fac, Dept Biochem, Konya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Dursun, Sukru</creatorName>
      <givenName>Sukru</givenName>
      <familyName>Dursun</familyName>
      <affiliation>Aksaray Univ, Vet Fac, Dept Gynecol &amp; Obstet, Aksaray, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Cryopreservation Effects On Ram Sperm Ultrastructure</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2020</publicationYear>
  <dates>
    <date dateType="Issued">2020-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/5717</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1089/bio.2020.0056</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Cryoprotectants are known to have protective effects against cryodamage to spermatozoa. In this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and cryoprotectants/trehalose combinations on frozen-thawed ram spermatozoa were investigated at the ultrastructural level. For this purpose, ejaculates collected from Konya Merino rams were pooled and diluted with a tris-based extender containing additives, including 5% glycerol, 3% glycerol +60 mM trehalose, 1.5% glycerol +100 mM trehalose, 5% ethylene glycol, 3% ethylene glycol +60 mM trehalose, and 1.5% ethylene glycol +100 mM trehalose. They were all cooled to 5 degrees C and then frozen in 0.25 mL French straws in liquid nitrogen. The samples were thawed at 37 degrees C and centrifuged to remove the diluents. Then, they were processed using a scanning transmission electron microscope. In the statistical analysis, the number of ultrastructurally cryodamaged and intact spermatozoa were counted in longitudinal and transverse ultrathin sections in all groups by electron microscopic examination. The amount of intact spermatozoa in the groups containing 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose was found to be higher than other groups (p &amp;lt; 0.05). As a result, it was suggested that the groups of 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose provided the highest protection for the ultrastructural morphology of frozen-thawed Konya Merino ram spermatozoa among the groups.</description>
  </descriptions>
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