1 Ocak 2016 Dergi makalesi Açık Erişim

Demir, Bilal; Yilmaz, Tulay; Guler, Emine; Gumus, Z. Pinar; Akbulut, Huseyin; Aldemir, Ebru; Coskunol, Hakan; Colak, Demet Goen; Cianga, Ioan; Yamada, Shuhei; Timur, Suna; Endo, Takeshi; Yagci, Yusuf

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/53755</identifier>
  <creators>
    <creator>
      <creatorName>Demir, Bilal</creatorName>
      <givenName>Bilal</givenName>
      <familyName>Demir</familyName>
      <affiliation>Ege Univ, Dept Biochem, Fac Sci, TR-35100 Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yilmaz, Tulay</creatorName>
      <givenName>Tulay</givenName>
      <familyName>Yilmaz</familyName>
    </creator>
    <creator>
      <creatorName>Guler, Emine</creatorName>
      <givenName>Emine</givenName>
      <familyName>Guler</familyName>
    </creator>
    <creator>
      <creatorName>Gumus, Z. Pinar</creatorName>
      <givenName>Z. Pinar</givenName>
      <familyName>Gumus</familyName>
      <affiliation>Ege Univ, Inst Drug Abuse Toxicol &amp; Pharmaceut Sci, TR-35100 Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Akbulut, Huseyin</creatorName>
      <givenName>Huseyin</givenName>
      <familyName>Akbulut</familyName>
      <affiliation>Istanbul Tech Univ, Dept Chem, Fac Sci &amp; Letters, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Aldemir, Ebru</creatorName>
      <givenName>Ebru</givenName>
      <familyName>Aldemir</familyName>
      <affiliation>Ege Univ, Inst Drug Abuse Toxicol &amp; Pharmaceut Sci, TR-35100 Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Coskunol, Hakan</creatorName>
      <givenName>Hakan</givenName>
      <familyName>Coskunol</familyName>
      <affiliation>Ege Univ, Inst Drug Abuse Toxicol &amp; Pharmaceut Sci, TR-35100 Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Colak, Demet Goen</creatorName>
      <givenName>Demet Goen</givenName>
      <familyName>Colak</familyName>
      <affiliation>Istanbul Tech Univ, Dept Chem, Fac Sci &amp; Letters, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Cianga, Ioan</creatorName>
      <givenName>Ioan</givenName>
      <familyName>Cianga</familyName>
    </creator>
    <creator>
      <creatorName>Yamada, Shuhei</creatorName>
      <givenName>Shuhei</givenName>
      <familyName>Yamada</familyName>
      <affiliation>Kinki Univ, Mol Engn Inst, 11-6 Kayanomori, Iizuka, Fukuoka 8208555, Japan</affiliation>
    </creator>
    <creator>
      <creatorName>Timur, Suna</creatorName>
      <givenName>Suna</givenName>
      <familyName>Timur</familyName>
    </creator>
    <creator>
      <creatorName>Endo, Takeshi</creatorName>
      <givenName>Takeshi</givenName>
      <familyName>Endo</familyName>
      <affiliation>Kinki Univ, Mol Engn Inst, 11-6 Kayanomori, Iizuka, Fukuoka 8208555, Japan</affiliation>
    </creator>
    <creator>
      <creatorName>Yagci, Yusuf</creatorName>
      <givenName>Yusuf</givenName>
      <familyName>Yagci</familyName>
      <affiliation>Istanbul Tech Univ, Dept Chem, Fac Sci &amp; Letters, Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Polypeptide With Electroactive Endgroups As Sensing Platform For The Abused Drug 'Methamphetamine' By Bioelectrochemical Method</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2016</publicationYear>
  <dates>
    <date dateType="Issued">2016-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/53755</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.talanta.2016.09.042</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Affinity-type sensors have emerged as outstanding platforms in the detection of diagnostic protein markers, nucleic acids and drugs. Thus, these novel platforms containing antibodies could be integrated into the monitoring systems for abused drugs. Herein, we established a novel detection platform for the analysis of a common illicit drug; methamphetamine (METH). Initially, a fluorescent-labeled polypeptide (EDOT-BTDA-Pala), derived from L-alanine N-carboxyanhydride (L-Ala-NCA) via ring-opening polymerization using 4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)benzo[c][1,2,5]thiadiazole-5,6-diamine (EDOT-NH2-BTDA) as initiator, was employed as a glassy carbon electrode (GCE) covering host, in order to immobilize the METH selective antibody. Prior to the examination of analytical features, GCE/EDOT-BTDA-Pala/Antibody surface was successfully characterized in the way of electrochemical (cyclic voltammetry and electrochemical impedance spectroscopy) and microscopic techniques (scanning electron microscopy and fluorescence microscopy). As for the analytical characterization, linearity and limit of detection (LOD) were found as 10-100 mu g/mL with an equation of y=0.0429x-0.2347, (R-2=0.996) and 13.07 mu g/mL, respectively. Moreover, sample application using artificial urine, saliva and serum samples spiked with METH (10, 25, 50 mu g/mL) were performed and LC-MS/MS system was used for further confirmation. The described platform can be adapted to monitor the other types of abused drugs by using suitably selected biorecognition elements.</description>
  </descriptions>
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