1 Ocak 2017 Dergi makalesi Açık Erişim

Atalay, Pinar Buket; Asci, Oyku; Kaya, Fatih Oner; Tuna, Bilge Guvenc

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/52669</identifier>
  <creators>
    <creator>
      <creatorName>Atalay, Pinar Buket</creatorName>
      <givenName>Pinar Buket</givenName>
      <familyName>Atalay</familyName>
      <affiliation>Maltepe Univ, Dept Med Biol &amp; Genet, Fac Med, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Asci, Oyku</creatorName>
      <givenName>Oyku</givenName>
      <familyName>Asci</familyName>
      <affiliation>Maltepe Univ, Grad Sch Hlth Sci, Dept Clin Embryol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kaya, Fatih Oner</creatorName>
      <givenName>Fatih Oner</givenName>
      <familyName>Kaya</familyName>
      <affiliation>Maltepe Univ, Dept Internal Med, Fac Med, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Tuna, Bilge Guvenc</creatorName>
      <givenName>Bilge Guvenc</givenName>
      <familyName>Tuna</familyName>
      <affiliation>Yeditepe Univ, Sch Med, Dept Biophys, Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Hydrogen Peroxide Prolongs Mitotic Arrest In A Dose Dependent Manner And Independently Of The Spindle Assembly Checkpoint Activity In Saccharomyces Cerevisiae</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/52669</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1556/018.68.2017.4.12</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Oxidative stress and chromosome missegregation are important factors that are linked to aneuploidy. A major reason for chromosome missegragation is the inappropriate activity of the spindle assembly checkpoint (SAC), a conserved surveillance mechanism that monitors the state of kinetochore-microtubule attachments to ensure equal chromosome segregation in mitosis. SAC-activation induces a prolonged mitotic arrest. Mitosis is considered the most vulnerable cell cycle phase to several external signals, therefore increasing the time cells spent in this phase via mitotic arrest induction by SAC-activating agents is favorable for cancer therapy. Cancer cells also display elevated oxidative stress due to abnormally high production of reactive oxygen species (ROS). However, the effect of increased oxidative stress on the duration of mitotic arrest remains largely unknown. In this study, we investigated the effect of H2O2-induced oxidative stress on the mitotic arrest induced by a SAC-activating agent (nocodazole) in Saccharomyces cerevisiae. Our data suggest that oxidative stress prolongs SAC-activation induced mitotic arrest in a dose dependent manner. We, in addition, investigated the effect of H2O2 treatment on the mitotic arrest induced independently of SAC-activation by using a conditional mutant (cdc23) and showed that the effect of H2O2-induced oxidative stress on mitotic arrest is independent of the SAC activity.</description>
  </descriptions>
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