1 Ocak 2017 Dergi makalesi Açık Erişim

Schneider, Falk; Waithe, Dominic; Clausen, Mathias P.; Galiani, Silvia; Koller, Thomas; Ozhan, Gunes; Eggeling, Christian; Sezgin, Erdinc

DataCite XML

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/51337</identifier>
  <creators>
    <creator>
      <creatorName>Schneider, Falk</creatorName>
      <givenName>Falk</givenName>
      <familyName>Schneider</familyName>
      <affiliation>Univ Oxford, MRC Human Immunol Unit, Oxford OX3 9DS, England</affiliation>
    </creator>
    <creator>
      <creatorName>Waithe, Dominic</creatorName>
      <givenName>Dominic</givenName>
      <familyName>Waithe</familyName>
      <affiliation>Univ Oxford, Weatherall Inst Mol Med, Wolfson Imaging Ctr Oxford, Oxford OX3 9DS, England</affiliation>
    </creator>
    <creator>
      <creatorName>Clausen, Mathias P.</creatorName>
      <givenName>Mathias P.</givenName>
      <familyName>Clausen</familyName>
    </creator>
    <creator>
      <creatorName>Galiani, Silvia</creatorName>
      <givenName>Silvia</givenName>
      <familyName>Galiani</familyName>
      <affiliation>Univ Oxford, MRC Human Immunol Unit, Oxford OX3 9DS, England</affiliation>
    </creator>
    <creator>
      <creatorName>Koller, Thomas</creatorName>
      <givenName>Thomas</givenName>
      <familyName>Koller</familyName>
      <affiliation>Univ Oxford, MRC Human Immunol Unit, Oxford OX3 9DS, England</affiliation>
    </creator>
    <creator>
      <creatorName>Ozhan, Gunes</creatorName>
      <givenName>Gunes</givenName>
      <familyName>Ozhan</familyName>
    </creator>
    <creator>
      <creatorName>Eggeling, Christian</creatorName>
      <givenName>Christian</givenName>
      <familyName>Eggeling</familyName>
    </creator>
    <creator>
      <creatorName>Sezgin, Erdinc</creatorName>
      <givenName>Erdinc</givenName>
      <familyName>Sezgin</familyName>
      <affiliation>Univ Oxford, MRC Human Immunol Unit, Oxford OX3 9DS, England</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Diffusion Of Lipids And Gpi-Anchored Proteins In Actin-Free Plasma Membrane Vesicles Measured By Sted-Fcs</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/51337</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1091/mbc.E16-07-0536</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Diffusion and interaction dynamics of molecules at the plasma membrane play an important role in cellular signaling and are suggested to be strongly associated with the actin cytoskeleton. Here we use superresolution STED microscopy combined with fluorescence correlation spectroscopy (STED-FCS) to access and compare the diffusion characteristics of fluorescent lipid analogues and GPI-anchored proteins (GPI-APs) in the live-cell plasma membrane and in actin cytoskeleton-free, cell-derived giant plasma membrane vesicles (GPMVs). Hindered diffusion of phospholipids and sphingolipids is abolished in the GPMVs, whereas transient nanodomain incorporation of ganglioside lipid GM1 is apparent in both the live-cell membrane and GPMVs. For GPI-APs, we detect two molecular pools in living cells; one pool shows high mobility with transient incorporation into nanodomains, and the other pool forms immobile clusters, both of which disappear in GPMVs. Our data underline the crucial role of the actin cortex in maintaining hindered diffusion modes of many but not all of the membrane molecules and highlight a powerful experimental approach to decipher specific influences on molecular plasma membrane dynamics.</description>
  </descriptions>
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