1 Ocak 2017 Dergi makalesi Açık Erişim

Orel, Didem Canik; Reid, Cheryl L.; Fuchs, Marc; Burr, Thomas J.

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/51017</identifier>
  <creators>
    <creator>
      <creatorName>Orel, Didem Canik</creatorName>
      <givenName>Didem Canik</givenName>
      <familyName>Orel</familyName>
      <affiliation>Ankara Univ, Dept Plant Protect, Fac Agr, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Reid, Cheryl L.</creatorName>
      <givenName>Cheryl L.</givenName>
      <familyName>Reid</familyName>
      <affiliation>New York State Agr Exptl Stn, Sch Integrat Plant Sci, Plant Pathol &amp; Plant Microbe Biol Sect, Geneva, NY 14456 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Fuchs, Marc</creatorName>
      <givenName>Marc</givenName>
      <familyName>Fuchs</familyName>
      <affiliation>New York State Agr Exptl Stn, Sch Integrat Plant Sci, Plant Pathol &amp; Plant Microbe Biol Sect, Geneva, NY 14456 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Burr, Thomas J.</creatorName>
      <givenName>Thomas J.</givenName>
      <familyName>Burr</familyName>
      <affiliation>New York State Agr Exptl Stn, Sch Integrat Plant Sci, Plant Pathol &amp; Plant Microbe Biol Sect, Geneva, NY 14456 USA</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Identifying Environmental Sources Of Agrobacterium Vitis In Vineyards And Wild Grapevines</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/51017</alternateIdentifier>
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  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.5344/ajev.2016.16085</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Agrobacterium vitis, the primary cause of grape crown gall disease, is known to survive internally in grapevines and to spread in propagation material. In this study, we showed that the bacterium can be detected in dormant grape buds and on surfaces of leaves collected from commercial vineyards. Using a highly selective and sensitive method based on magnetic capture hybridization (MCH) together with real-time PCR, we detected A. vitis in as much as 90% of dormant bud samples and in up to 40% of leaf samples from individual vineyards. The highest percentages of detection occurred in samples collected from vineyards with high incidences of crown gall. A. vitis was also detected in 22% of wild grapevines (Vitis riparia) collected in New York and in 25% of feral grapevines that included V. californica in California. Several of these vines were growing more than 2 km from commercial vineyards, demonstrating that wild grapevines can serve as a significant inoculum reservoir. The specificity of the MCH and real-time PCR assay used to detect tumorigenic A. vitis in the environment was further demonstrated by the finding that 69 nontumorigenic strains from regions across the United States did not amplify a virD2 PCR product.</description>
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