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Screening, selection and real-time qPCR validation for phytoplasma resistance in sesame (Sesamum indicum L.)

Ustun, Rustem; Yol, Engin; Ikten, Cengiz; Catal, Mursel; Uzun, Bulent


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/43743</identifier>
  <creators>
    <creator>
      <creatorName>Ustun, Rustem</creatorName>
      <givenName>Rustem</givenName>
      <familyName>Ustun</familyName>
      <affiliation>Akdeniz Univ, Dept Field Crops, Fac Agr, TR-07058 Antalya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yol, Engin</creatorName>
      <givenName>Engin</givenName>
      <familyName>Yol</familyName>
      <affiliation>Akdeniz Univ, Dept Field Crops, Fac Agr, TR-07058 Antalya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ikten, Cengiz</creatorName>
      <givenName>Cengiz</givenName>
      <familyName>Ikten</familyName>
      <affiliation>Akdeniz Univ, Dept Plant Protect, Fac Agr, Antalya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Catal, Mursel</creatorName>
      <givenName>Mursel</givenName>
      <familyName>Catal</familyName>
      <affiliation>Akdeniz Univ, Dept Plant Protect, Fac Agr, Antalya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Uzun, Bulent</creatorName>
      <givenName>Bulent</givenName>
      <familyName>Uzun</familyName>
      <affiliation>Akdeniz Univ, Dept Field Crops, Fac Agr, TR-07058 Antalya, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Screening, Selection And Real-Time Qpcr Validation For Phytoplasma Resistance In Sesame (Sesamum Indicum L.)</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/43743</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s10681-017-1936-y</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Phyllody is one of the most destructive diseases of sesame and causes serious yield losses worldwide. The present research was conducted to identify phyllody resistant genotypes in sesame. A total of 542 sesame genotypes were screened for the disease resistance in the field using a disease incidence scale of 1-5 in the year 2012. Three hundred four genotypes showing high disease intensity were eliminated under artificially infected field conditions. In the year 2013, only 30 out of 238 accessions were determined as potential resistant genotypes based on the disease incidence scale. These selected genotypes were further evaluated for confirmation of the resistance in greenhouse conditions using the phytoplasma-infected vector insects under choice and no-choice conditions. Furthermore, real-time qPCR was employed for detection and quantification of phytoplasmas to select true resistant genotypes. The sesame accessions ACS38 and ACS102 were identified as resistant to the disease after evaluation in field, greenhouse and qPCR assays. This work is one of the most comprehensive studies to select genotypes resistant to the diseases caused by phytoplasmas.</description>
  </descriptions>
</resource>
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