1 Ocak 2009 Dergi makalesi Açık Erişim

Oguzoglu, T. C.; Tan, M. T.; Toplu, N.; Demir, A. B.; Bilge-Dagalp, S.; Karaoglu, T.; Ozkul, A.; Alkan, F.; Burgu, I.; Haas, L.; Greiser-Wilke, I.

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    <subfield code="a">Blood samples from sheep and/or goats from eight small ruminant flocks in the Turkish provinces of Aydin and Burdur were tested for the presence of Pestiviruses using an antigen-capture ELISA. From clinically affected animals, pathological and immunohistochemical findings were recorded. Post mortem examination of a virus-positive lamb showing abnormal fleece and paralysis of the hind legs revealed nonsuppurative meningoencephalomyelitis with hypomyelinogenesis. By immunohistochemistry Pestivirus antigen was detected in ail parts of the brain including cerebellum, cerebral hemispheres and midbrain. Two Pestivirus isolates from a sheep and a goat kid, respectively, were isolated from samples that were positive in the antigen-capture ELISA. Genetic typing using the 5'-NTR (288 bp) and N-pro (738 bp) showed that both were Border disease virus (BDV) isolates. By phylogenetic analysis, they formed a cluster clearly separated from the known clusters BDV-1 to BDV-6 and might therefore represent a new Subgroup (BDV-7?). This is the first report confirming the occurrence and partial characterisation of BDV infection in small ruminants in Turkey. (C) 2008 Elsevier B.V. All rights reserved.</subfield>
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