1 Ocak 2018 Dergi makalesi Açık Erişim

Kose, Sevil; Aerts-Kaya, Fatima; Kopru, Cagla Zubeyde; Nemutlu, Emirhan; Koskonmaz, Baris; Karaosmanoglu, Beren; Taskiran, Ekim Zihni; Altun, Belgin; Cetinkaya, Duygu Uckan; Korkusuz, Petek

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/34355</identifier>
  <creators>
    <creator>
      <creatorName>Kose, Sevil</creatorName>
      <givenName>Sevil</givenName>
      <familyName>Kose</familyName>
      <affiliation>Hacettepe Univ, Ctr Stem Cell Res &amp; Dev PEDI STEM, Inst Hlth Sci, Dept Stem Cell Sci, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Aerts-Kaya, Fatima</creatorName>
      <givenName>Fatima</givenName>
      <familyName>Aerts-Kaya</familyName>
      <affiliation>Hacettepe Univ, Ctr Stem Cell Res &amp; Dev PEDI STEM, Inst Hlth Sci, Dept Stem Cell Sci, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kopru, Cagla Zubeyde</creatorName>
      <givenName>Cagla Zubeyde</givenName>
      <familyName>Kopru</familyName>
      <affiliation>Yuksek Ihtisas Univ, Fac Med, Dept Histol &amp; Embryol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Nemutlu, Emirhan</creatorName>
      <givenName>Emirhan</givenName>
      <familyName>Nemutlu</familyName>
      <affiliation>Hacettepe Univ, Fac Pharm, Dept Analyt Chem, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Koskonmaz, Baris</creatorName>
      <givenName>Baris</givenName>
      <familyName>Koskonmaz</familyName>
      <affiliation>Hacettepe Univ, Childrens Hosp, BMT Unit, Pediat Hematol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Karaosmanoglu, Beren</creatorName>
      <givenName>Beren</givenName>
      <familyName>Karaosmanoglu</familyName>
      <affiliation>Hacettepe Univ, Ctr Stem Cell Res &amp; Dev PEDI STEM, Inst Hlth Sci, Dept Stem Cell Sci, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Taskiran, Ekim Zihni</creatorName>
      <givenName>Ekim Zihni</givenName>
      <familyName>Taskiran</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Med Genet, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Altun, Belgin</creatorName>
      <givenName>Belgin</givenName>
      <familyName>Altun</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Microbiol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Cetinkaya, Duygu Uckan</creatorName>
      <givenName>Duygu Uckan</givenName>
      <familyName>Cetinkaya</familyName>
    </creator>
    <creator>
      <creatorName>Korkusuz, Petek</creatorName>
      <givenName>Petek</givenName>
      <familyName>Korkusuz</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Histol &amp; Embryol, TR-06100 Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Human Bone Marrow Mesenchymal Stem Cells Secrete Endocannabinoids That Stimulate In Vitro Hematopoietic Stem Cell Migration Effectively Comparable To Beta-Adrenergic Stimulation</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2018</publicationYear>
  <dates>
    <date dateType="Issued">2018-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/34355</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.exphem.2017.09.009</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Granulocyte colony-stimulating factor (G-CSF) is a well-known hematopoietic stem cell (HSC)mobilizing agent used in both allogeneic and autologous transplantation. However, a proportion of patients or healthy donors fail to mobilize a sufficient number of cells. New mobilization agents are therefore needed. Endocannabinoids (eCBs) are endogenous lipid mediators generated in the brain and peripheral tissues and activate the cannabinoid receptors CB1 and CB2. We suggest that eCBs may act as mobilizers of HSCs from the bone marrow (BM) under stress conditions as beta-adrenergic receptors (Adr beta). This study demonstrates that BM mesenchymal stem cells (MSCs) secrete anandamide (AEA) and 2-arachidonylglycerol (2-AG) and the peripheral blood (PB) and BM microenvironment contain AEA and 2-AG. 2-AG levels are significantly higher in PB of the G-CSF-treated group compared with BM plasma. BM mononuclear cells (MNCs) and CD34 HSCs express CB1, CB2, and Adr beta subtypes. CD34+ HSCs had higher CB1 and CB2 receptor expression in G-CSF-untreated and G-CSF-treated groups compared with MSCs. MNCs but not MSCs expressed CB1 and CB2 receptors based on qRT-PCR and flow cytometry. AEA- and 2-AG-stimulated HSC migration was blocked by eCB receptor antagonists in an in vitro migration assay. In conclusion, components of the eCB system and their interaction with Adr beta subtypes were demonstrated on HSCs and MSCs of G-CSF-treated and G-CSF-untreated healthy donors in vitro, revealing that eCBs might be potential candidates to enhance or facilitate G-CSF-mediated HSC migration under stress conditions in a clinical setting. (C) 2018 ISEH Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.</description>
  </descriptions>
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