1 Ocak 2018 Dergi makalesi Açık Erişim

Yildiz-Ozer, Merve; Oztopcu-Vatan, Pinar; Kus, Gokhan

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/33497</identifier>
  <creators>
    <creator>
      <creatorName>Yildiz-Ozer, Merve</creatorName>
      <givenName>Merve</givenName>
      <familyName>Yildiz-Ozer</familyName>
      <affiliation>Eskisehir Osmangazi Univ, Grad Sch Nat &amp; Appl Sci, Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Oztopcu-Vatan, Pinar</creatorName>
      <givenName>Pinar</givenName>
      <familyName>Oztopcu-Vatan</familyName>
      <affiliation>Eskisehir Osmangazi Univ, Fac Arts &amp; Sci, Dept Biol, Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kus, Gokhan</creatorName>
      <givenName>Gokhan</givenName>
      <familyName>Kus</familyName>
      <affiliation>Anadolu Univ, Open Fac, Dept Hlth Programme, Eskisehir, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>The Investigation Of Ceranib-2 On Apoptosis And Drug Interaction With Carboplatin In Human Non Small Cell Lung Cancer Cells In Vitro</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2018</publicationYear>
  <dates>
    <date dateType="Issued">2018-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/33497</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s10616-017-0154-8</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Ceramide is found to be involved in inhibition of cell division and induction of apoptosis in certain tumour cells. Ceranib-2 is an agent that increases ceramide levels by inhibiting ceramidase in cancer cells. Therefore, we aimed to investigate the effects of ceranib-2 on cell survival, apoptosis and interaction with carboplatin in human non-small cell lung cancer cells. The cytotoxic effect of ceranib-2 (1-100 A mu M) was determined by MTT assay in human lung adenocarcinoma (A549) and large cell lung carcinoma (H460) cells. Carboplatin (1-100 A mu M) and lung bronchial epithelial cells (BEAS-2B) were used as positive controls. Morphological and ultrastructural changes were analysed by light microscope and TEM. Apoptotic/necrotic cell death and acid ceramidase activity were analysed by ELISA. Combination effects of ceranib-2 and carboplatin were investigated by MTT. The expression levels of CASP3, CASP9, BAX and BCL-2 were examined by qRT-PCR. The IC50 of ceranib-2 was determined as 22 mu M in A549 cells and 8 mu M in H460 cells for 24 h. Morphological changes and induction of DNA fragmentation have revealed apoptotic effects of ceranib-2 in both cell lines. Ceranib-2 and carboplatin has shown synergism in combined treatment at 10 and 25 mu M doses in H460 cells for 24 h. Ceranib-2 inhibited acid ceramidase activity by 44% at 25 A mu M in H460 cells. Finally, CASP3, CASP9 and BAX expressions were increased while BCL-2 expression was reduced in both cells. Our results obtained some preliminary results about the cytotoxic and apoptotic effects of ceranib-2 for the first time in NSCLC cell lines.</description>
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