1 Ocak 2018 Dergi makalesi Açık Erişim

Akkaya, Ozlem; Perez-Pantoja, Danilo R.; Calles, Belen; Nikel, Pablo I.; de Lorenzo, Victor

JSON

{
  "conceptrecid": "30470", 
  "created": "2021-03-15T18:15:07.447125+00:00", 
  "doi": "10.1128/mBio.01512-18", 
  "files": [
    {
      "bucket": "93d398fc-1918-4abc-a3fd-33e90b505051", 
      "checksum": "md5:abdbf61a304b5507d7737d7a7a802b2e", 
      "key": "bib-b324ef41-c505-405d-a95a-4a04ebdcef49.txt", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/93d398fc-1918-4abc-a3fd-33e90b505051/bib-b324ef41-c505-405d-a95a-4a04ebdcef49.txt"
      }, 
      "size": 197, 
      "type": "txt"
    }
  ], 
  "id": 30471, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.1128/mBio.01512-18.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/93d398fc-1918-4abc-a3fd-33e90b505051", 
    "doi": "https://doi.org/10.1128/mBio.01512-18", 
    "html": "https://aperta.ulakbim.gov.tr/record/30471", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/30471", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/30471"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-destekli-proje-yayinlari"
      }
    ], 
    "creators": [
      {
        "affiliation": "Gebze Tech Univ, Fac Sci, Dept Mol Biol & Genet, Kocaeli, Turkey", 
        "name": "Akkaya, Ozlem"
      }, 
      {
        "affiliation": "Univ Tecnol Metropolitana, Programa Inst Fomento Invest Desarrollo & Innovac, Santiago, Chile", 
        "name": "Perez-Pantoja, Danilo R."
      }, 
      {
        "affiliation": "Ctr Nacl Biotecnol, Syst & Synthet Biol Program, Madrid, Spain", 
        "name": "Calles, Belen"
      }, 
      {
        "affiliation": "Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Lyngby, Denmark", 
        "name": "Nikel, Pablo I."
      }, 
      {
        "affiliation": "Ctr Nacl Biotecnol, Syst & Synthet Biol Program, Madrid, Spain", 
        "name": "de Lorenzo, Victor"
      }
    ], 
    "description": "During evolution of biodegradation pathways for xenobiotic compounds involving Rieske nonheme iron oxygenases, the transition toward novel substrates is frequently associated with faulty reactions. Such events release reactive oxygen species (ROS), which are endowed with high mutagenic potential. In this study, we evaluated how the operation of the background metabolic network by an environmental bacterium may either foster or curtail the still-evolving pathway for 2,4-dinitrotoluene (2,4-DNT) catabolism. To this end, the genetically tractable strain Pseudomonas putida EM173 was implanted with the whole genetic complement necessary for the complete biodegradation of 2,4-DNT (recruited from the environmental isolate Burkholderia sp. R34). By using reporter technology and direct measurements of ROS formation, we observed that the engineered P. putida strain experienced oxidative stress when catabolizing the nitroaromatic substrate. However, the formation of ROS was neither translated into significant activation of the SOS response to DNA damage nor did it result in a mutagenic regime (unlike what has been observed in Burkholderia sp. R34, the original host of the pathway). To inspect whether the tolerance of P. putida to oxidative challenges could be traced to its characteristic reductive redox regime, we artificially altered the NAD(P)H pool by means of a water-forming, NADH-specific oxidase. Under the resulting low-NAD(P)H status, catabolism of 2,4-DNT triggered a conspicuous mutagenic and genomic diversification scenario. These results indicate that the background biochemical network of environmental bacteria ultimately determines the evolvability of metabolic pathways. Moreover, the data explain the efficacy of some bacteria (e.g., pseudomonads) to host and evolve with new catabolic routes.", 
    "doi": "10.1128/mBio.01512-18", 
    "has_grant": false, 
    "journal": {
      "issue": "4", 
      "title": "MBIO", 
      "volume": "9"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2018-01-01", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "30471"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "30470"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "title": "The Metabolic Redox Regime of Pseudomonas putida Tunes Its Evolvability toward Novel Xenobiotic Substrates"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 9.0, 
    "unique_downloads": 9.0, 
    "unique_views": 24.0, 
    "version_downloads": 9.0, 
    "version_unique_downloads": 9.0, 
    "version_unique_views": 24.0, 
    "version_views": 25.0, 
    "version_volume": 1773.0, 
    "views": 25.0, 
    "volume": 1773.0
  }, 
  "updated": "2021-03-15T18:15:07.499117+00:00"
}