3 Ağustos 2025 Dergi makalesi Ambargolu Erişim

Ali Hayatu; Esra Nur Akyıldız; Betül Karabudak; Sena Aksoy; Harun Erol; Bekir Salih; Hacı Mehmet KAYILI

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  <identifier identifierType="DOI">10.48623/aperta.285958</identifier>
  <creators>
    <creator>
      <creatorName>Ali Hayatu</creatorName>
      <affiliation>Karabük Üniversitesi</affiliation>
    </creator>
    <creator>
      <creatorName>Esra Nur Akyıldız</creatorName>
      <affiliation>Karabük Üniversitesi</affiliation>
    </creator>
    <creator>
      <creatorName>Betül Karabudak</creatorName>
      <affiliation>Karabük Üniversitesi</affiliation>
    </creator>
    <creator>
      <creatorName>Sena Aksoy</creatorName>
      <affiliation>Karabük Üniversitesi</affiliation>
    </creator>
    <creator>
      <creatorName>Harun Erol</creatorName>
      <affiliation>Karabük Üniversitesi</affiliation>
    </creator>
    <creator>
      <creatorName>Bekir Salih</creatorName>
      <affiliation>Hacettepe  Üniversitesi</affiliation>
    </creator>
    <creator>
      <creatorName>Hacı Mehmet KAYILI</creatorName>
      <affiliation>Karabük Üniversitesi</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Glycosylation Signatures Of Thyroglobulin In Papillary Thyroid Carcinoma</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2025</publicationYear>
  <subjects>
    <subject>papillary thyroid cancer,</subject>
    <subject>N-glycosylation</subject>
    <subject>thyroglobulin</subject>
    <subject>mass spectrometry</subject>
  </subjects>
  <dates>
    <date dateType="Available">2026-01-01</date>
    <date dateType="Accepted">2025-08-03</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/285958</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.48623/aperta.285957</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by-sa">Creative Commons Attribution Share-Alike</rights>
    <rights rightsURI="info:eu-repo/semantics/embargoedAccess">Embargoed Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Thyroglobulin (Tg) is a clinically established biomarker for thyroid cancer; however, its diagnostic specificity remains limited due to confounding benign conditions. &amp;nbsp;This study investigated changes in the site-specific &lt;em&gt;N-&lt;/em&gt;glycosylation profiles of the thyroglobulin protein derived from papillary thyroid cancerous and adjacent healthy tissues. Initially, paraffin was removed from the tissues, and the extracted proteins were subjected to proteolytic digestion into peptides. The generated peptides were analyzed using nano-liquid chromatography with tandem mass spectrometry (nLC-MS/MS). Nine distinct &lt;em&gt;N-&lt;/em&gt;glycosylation sites were identified and quantified to determine the differences between the cancerous and the healthy tissue samples. Statistical tests were performed to evaluate the differences in glycoform between these groups. The glycoforms HexNAc(3)Hex(5) and HexNAc(3)Hex(6) at the Asn1365 site demonstrated notable discriminatory potential between cancerous and healthy tissues. The results of receiver operating characteristic (ROC) analysis suggest that these glycoforms may serve as promising biomarker candidates. This study reveals that alterations in the relative abundance of specific glycoforms at defined glycosylation sites of the thyroglobulin protein may hold biomarker potential specific to thyroid cancer, providing a foundation for future research.&lt;/p&gt;</description>
    <description descriptionType="Other">This work was supported by a grant from the Health Institutes of Türkiye (TUSEB) with a grant number of 28731.</description>
  </descriptions>
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