1 Ocak 2023 Dergi makalesi Açık Erişim

Turkoglu, Aras; Haliloglu, Kamil; Tosun, Metin; Szulc, Piotr; Demirel, Fatih; Eren, Baris; Bujak, Henryk; Karagoz, Halit; Selwet, Marek; Ozkan, Guller; Niedbala, Gniewko

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    "creators": [
      {
        "affiliation": "Necmettin Erbakan Univ, Fac Agr, Dept Field Crops, TR-42310 Konya, Turkiye", 
        "name": "Turkoglu, Aras"
      }, 
      {
        "affiliation": "Ataturk Univ, Fac Agr, Dept Field Crops, TR-25240 Erzurum, Turkiye", 
        "name": "Haliloglu, Kamil"
      }, 
      {
        "affiliation": "Ataturk Univ, Fac Agr, Dept Field Crops, TR-25240 Erzurum, Turkiye", 
        "name": "Tosun, Metin"
      }, 
      {
        "affiliation": "Poznan Univ Life Sci, Dept Agron, Dojazd 11, PL-60632 Poznan, Poland", 
        "name": "Szulc, Piotr"
      }, 
      {
        "affiliation": "Igdir Univ, Fac Agr, Dept Agr Biotechnol, TR-76000 Igdir, Turkiye", 
        "name": "Demirel, Fatih"
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      {
        "affiliation": "Igdir Univ, Fac Agr, Dept Agr Biotechnol, TR-76000 Igdir, Turkiye", 
        "name": "Eren, Baris"
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      {
        "name": "Bujak, Henryk"
      }, 
      {
        "affiliation": "East Anatolia Agr Res Inst, TR-25240 Erzurum, Turkiye", 
        "name": "Karagoz, Halit"
      }, 
      {
        "affiliation": "Poznan Univ Life Sci, Dept Soil Sci & Microbiol, Szydlowska 50, PL-60656 Poznan, Poland", 
        "name": "Selwet, Marek"
      }, 
      {
        "affiliation": "Ankara Univ, Fac Sci, Dept Biol, TR-06100 Ankara, Turkiye", 
        "name": "Ozkan, Guller"
      }, 
      {
        "affiliation": "Poznan Univ Life Sci, Fac Environm & Mech Engn, Dept Biosyst Engn, Wojska Polskiego 50, PL-60627 Poznan, Poland", 
        "name": "Niedbala, Gniewko"
      }
    ], 
    "description": "<p>Wheat, which is scientifically known as Triticum aestivum L., is a very nutritious grain that serves as a key component of the human diet. The use of mutation breeding as a tool for crop improvement is a reasonably rapid procedure, and it generates a variety that may be used in selective breeding programs as well as functional gene investigations. The present experiment was used to evaluate the potential application of a conventional chemical mutagenesis technique via sodium azide (NaN3) for the germination and seedling growth stage in wheat. Experiments with NaN3 mutagenesis were conducted using four different treatment periods (0, 1, 2, and 3 h) and five different concentrations (0, 0.5, 1, 1.5, and 2 mM). The genomic instability and cytosine methylation of wheat using its seeds were investigated after they were treated. In order to evaluate the genomic instability and cytosine methylation in wheat that had been treated, interprimer binding site (iPBS) markers were used. The mutagenic effects of NaN3 treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat plants. The results of the experiment showed considerable changes in the iPBS profiles produced by the administration of the same treatments at different dosages and at different times. Coupled restriction enzyme digestion interprimer binding site (CRED-iPBS) assays identified changes in gDNA cytosine methylation. The highest polymorphism value was obtained during 1 h + 2 mM NaN3, while the lowest (20.7%) was obtained during 1 h + 1.5 mM NaN3. Results showed that treatments with NaN3 had an effect on the level of cytosine methylation and the stability of the genomic template in wheat plants in the germination stage. Additionally, an integrated method can be used to for mutation-assisted breeding using a molecular marker system in wheat followed by the selection of desired mutants.</p>", 
    "doi": "10.3390/agriculture13061242", 
    "has_grant": false, 
    "journal": {
      "issue": "6", 
      "pages": "15", 
      "title": "AGRICULTURE-BASEL", 
      "volume": "13"
    }, 
    "license": {
      "id": "cc-by"
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    "publication_date": "2023-01-01", 
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      "title": "Dergi makalesi", 
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    "science_branches": [
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    "title": "Sodium Azide as a Chemical Mutagen in Wheat (<i>Triticum aestivum</i> L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques"
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