1 Ocak 2023 Dergi makalesi Açık Erişim

Turkoglu, Aras; Haliloglu, Kamil; Tosun, Metin; Bujak, Henryk; Eren, Baris; Demirel, Fatih; Szulc, Piotr; Karagoz, Halit; Selwet, Marek; Ozkan, Guller; Niedbala, Gniewko

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{
  "@context": "https://schema.org/", 
  "@id": 266282, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Necmettin Erbakan Univ, Fac Agr, Dept Field Crops, TR-42310 Konya, Turkiye", 
      "name": "Turkoglu, Aras"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ataturk Univ, Fac Agr, Dept Field Crops, TR-25240 Erzurum, Turkiye", 
      "name": "Haliloglu, Kamil"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ataturk Univ, Fac Agr, Dept Field Crops, TR-25240 Erzurum, Turkiye", 
      "name": "Tosun, Metin"
    }, 
    {
      "@type": "Person", 
      "name": "Bujak, Henryk"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Igdir Univ, Fac Agr, Dept Agr Biotechnol, TR-76000 Igdir, Turkiye", 
      "name": "Eren, Baris"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Igdir Univ, Fac Agr, Dept Agr Biotechnol, TR-76000 Igdir, Turkiye", 
      "name": "Demirel, Fatih"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Poznan Univ Life Sci, Dept Agron, Dojazd 11, PL-60632 Poznan, Poland", 
      "name": "Szulc, Piotr"
    }, 
    {
      "@type": "Person", 
      "affiliation": "East Anatolia Agr Res Inst, TR-25240 Erzurum, Turkiye", 
      "name": "Karagoz, Halit"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Poznan Univ Life Sci, Dept Soil Sci & Microbiol, Szydlowska 50, PL-60656 Poznan, Poland", 
      "name": "Selwet, Marek"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Sci, Dept Biol, TR-06100 Ankara, Turkiye", 
      "name": "Ozkan, Guller"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Poznan Univ Life Sci, Fac Environm & Mech Engn, Dept Biosyst Engn, Wojska Polskiego 50, PL-60627 Poznan, Poland", 
      "name": "Niedbala, Gniewko"
    }
  ], 
  "datePublished": "2023-01-01", 
  "description": "<p>The use of mutagens in plant breeding is used to create new germplasm, increase agricultural yield, quality, and resistance to diseases and pests. Mutagens are physical or chemical factors that can alter the DNA or RNA structure of an organism, causing mutations above the expected level. One of the most common and potent chemical mutagens is EMS (ethyl-methane sulfonate), which produces point mutations in plants, but to a lesser degree can also cause the loss or deletion of a chromosomal region. This study used inter-primer binding site (iPBS) and coupled restriction enzyme digestion inter-primer binding site (CRED-iPBS) technique analysis to determine the effect of EMS mutagens on methylation rates in wheat genotypes at seedling growth stage. Treatments with five different EMS concentrations (0%; control, 0.1%, 0.2%, 0.3%, and 0.4%) at four different times (0; control, 3, 6, and 9 h) were used. Inter-primer binding site (iPBS) markers were employed to assess genomic instability and cytosine methylation in treated wheat. In seeds treated with EMS at different concentrations and times, the disappearance of regular bands and the formation of new bands due to the effects of the EMS mutagen revealed that genetic diversity exists. The CRED-iPBS analysis revealed that the 3 h + 0.1% EMS treatment produced the highest MspI polymorphism value (19.60%), while the 9 h + 0.1% EMS treatment produced the lowest value (10.90%). The mutagenic effects of EMS treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat. According to the current research, EMS mutagenesis may be a practical method for accelerating breeding programs to produce enough genetic diversity in wheat populations. Mutation-assisted breeding and the subsequent selection of desirable mutants using genetic markers may also be carried out in wheat utilizing an integrated strategy.</p>", 
  "headline": "Ethyl Methanesulfonate (EMS) Mutagen Toxicity-Induced DNA Damage, Cytosine Methylation Alteration, and iPBS-Retrotransposon Polymorphisms in Wheat (<i>Triticum aestivum</i> L.)", 
  "identifier": 266282, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Ethyl Methanesulfonate (EMS) Mutagen Toxicity-Induced DNA Damage, Cytosine Methylation Alteration, and iPBS-Retrotransposon Polymorphisms in Wheat (<i>Triticum aestivum</i> L.)", 
  "url": "https://aperta.ulakbim.gov.tr/record/266282"
}