1 Ocak 2022 Dergi makalesi Açık Erişim

Yuksel Egrilmez, Mehtap; Kocturk, Semra; Aktan, Sebnem; Oktay, Gulgun; Resmi, Halil; Simsek Keskin, Hatice; Guner Akdogan, Gul; Ozkan, Sebnem

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    "creators": [
      {
        "affiliation": "Dokuz Eylul Univ, Dept Mol Med, Inst Hlth Sci, TR-35340 Izmir, Turkey", 
        "name": "Yuksel Egrilmez, Mehtap"
      }, 
      {
        "affiliation": "Dokuz Eylul Univ, Fac Med, Dept Biochem, TR-35340 Izmir, Turkey", 
        "name": "Kocturk, Semra"
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      {
        "affiliation": "Dokuz Eylul Univ, Fac Med, Dept Dermatol & Venereal Dis, TR-35340 Izmir, Turkey", 
        "name": "Aktan, Sebnem"
      }, 
      {
        "affiliation": "Dokuz Eylul Univ, Fac Med, Dept Biochem, TR-35340 Izmir, Turkey", 
        "name": "Oktay, Gulgun"
      }, 
      {
        "affiliation": "Dokuz Eylul Univ, Fac Med, Dept Biochem, TR-35340 Izmir, Turkey", 
        "name": "Resmi, Halil"
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      {
        "affiliation": "Dokuz Eylul Univ, Fac Med, Dept Publ Hlth, TR-35340 Izmir, Turkey", 
        "name": "Simsek Keskin, Hatice"
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      {
        "name": "Guner Akdogan, Gul"
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      {
        "affiliation": "Dokuz Eylul Univ, Fac Med, Dept Dermatol & Venereal Dis, TR-35340 Izmir, Turkey", 
        "name": "Ozkan, Sebnem"
      }
    ], 
    "description": "Exposure to ultraviolet (UV) irradiation causes damage to the skin and induces photoaging. UV irradiation stimulates production of reactive oxygen/nitrogen species, which results in activation of epidermal growth factor receptor (EGFR) and mitogen-activated protein kinases (MAPK) in fibroblasts. MAPKs are responsible for activation of activator protein-1 (AP-1), which subsequently upregulates expression of matrix metalloproteinases (MMPs). Melatonin is a potent free radical scavenger which is known to have photoprotective effects. The aim of this study is to investigate the underlying molecular mechanisms for the photoprotective effects of melatonin in UVB-irradiated primary human dermal fibroblasts (HDFs) in terms of EGFR activation, oxidative/nitrosative damage, JNK/AP-1 activation, MMP activities, and the levels of tissue inhibitors of metalloproteinase-1 (TIMP-1) and type I procollagen (PIP-C). In this study, HDFs were pretreated with 1 mu M of melatonin and then irradiated with 0.1 J/cm(2) of UVB. Changes in the molecules were analyzed at different time points. Melatonin inhibited UVB-induced oxidative/nitrosative stress damage by reducing malondialdehyde, the ratio of oxidized/reduced glutathione, and nitrotyrosine. Melatonin downregulated UV-induced activation of EGFR and the JNK/AP-1 signaling pathway. UVB-induced activities of MMP-1 and MMP-3 were decreased and levels of TIMP-1 and PIP-C were increased by melatonin. These findings suggest that melatonin can protect against the adverse effects of UVB radiation by inhibiting MMP-1 and MMP-3 activity and increasing TIMP-1 and PIP-C levels, probably through the suppression of oxidative/nitrosative damage, EGFR, and JNK/AP-1 activation in HDFs.", 
    "doi": "10.3390/life12070950", 
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    "journal": {
      "issue": "7", 
      "title": "LIFE-BASEL", 
      "volume": "12"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2022-01-01", 
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    "resource_type": {
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      "title": "Dergi makalesi", 
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    "science_branches": [
      "Di\u011fer"
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    "title": "Melatonin Prevents UVB-Induced Skin Photoaging by Inhibiting Oxidative Damage and MMP Expression through JNK/AP-1 Signaling Pathway in Human Dermal Fibroblasts"
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