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Construction and Comparison of Trametes versicolor Laccase Biosensors Capable of Detecting Xenobiotics

Sezginturk, Mustafa Kemal; Odaci, Dilek; Pazarlioglu, Nurdan; Pilloton, Roberto; Dinckaya, Erhan; Telefoncu, Azmi; Timur, Suna


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/25039</identifier>
  <creators>
    <creator>
      <creatorName>Sezginturk, Mustafa Kemal</creatorName>
      <givenName>Mustafa Kemal</givenName>
      <familyName>Sezginturk</familyName>
      <affiliation>Namik Kemal Univ, Fac Arts &amp; Sci, Dept Chem, TR-59100 Tekirdag, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Odaci, Dilek</creatorName>
      <givenName>Dilek</givenName>
      <familyName>Odaci</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Pazarlioglu, Nurdan</creatorName>
      <givenName>Nurdan</givenName>
      <familyName>Pazarlioglu</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Pilloton, Roberto</creatorName>
      <givenName>Roberto</givenName>
      <familyName>Pilloton</familyName>
      <affiliation>ENEA CR Casaccia SP061, Rome, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Dinckaya, Erhan</creatorName>
      <givenName>Erhan</givenName>
      <familyName>Dinckaya</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Telefoncu, Azmi</creatorName>
      <givenName>Azmi</givenName>
      <familyName>Telefoncu</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Timur, Suna</creatorName>
      <givenName>Suna</givenName>
      <familyName>Timur</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, Bornova, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Construction And Comparison Of Trametes Versicolor Laccase Biosensors Capable Of Detecting Xenobiotics</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2010</publicationYear>
  <dates>
    <date dateType="Issued">2010-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/25039</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3109/10731191003776777</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Amperometric biosensors using laccase from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of 2,4-D in a real herbicide sample.</description>
  </descriptions>
</resource>
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