1 Ocak 2021 Dergi makalesi Açık Erişim

Onder, Seda; van Grol, Marco; Fidder, Alex; Xiao, Gaoping; Noort, Daan; Yerramalla, Udaya; Tacal, Ozden; Schopfer, Lawrence M.; Lockridge, Oksana

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/239402</identifier>
  <creators>
    <creator>
      <creatorName>Onder, Seda</creatorName>
      <givenName>Seda</givenName>
      <familyName>Onder</familyName>
      <affiliation>Hacettepe Univ, Sch Pharm, Dept Biochem, TR-06100 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>van Grol, Marco</creatorName>
      <givenName>Marco</givenName>
      <familyName>van Grol</familyName>
      <affiliation>TNO Org Appl Sci Res, NL-2280 AA Rijswijk, Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Fidder, Alex</creatorName>
      <givenName>Alex</givenName>
      <familyName>Fidder</familyName>
      <affiliation>TNO Org Appl Sci Res, NL-2280 AA Rijswijk, Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Xiao, Gaoping</creatorName>
      <givenName>Gaoping</givenName>
      <familyName>Xiao</familyName>
      <affiliation>Syd Labs Inc, Hopkinton, MA 01748 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Noort, Daan</creatorName>
      <givenName>Daan</givenName>
      <familyName>Noort</familyName>
      <affiliation>TNO Org Appl Sci Res, NL-2280 AA Rijswijk, Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Yerramalla, Udaya</creatorName>
      <givenName>Udaya</givenName>
      <familyName>Yerramalla</familyName>
      <affiliation>Precis Antibody, Columbia, MD 21045 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Tacal, Ozden</creatorName>
      <givenName>Ozden</givenName>
      <familyName>Tacal</familyName>
      <affiliation>Hacettepe Univ, Sch Pharm, Dept Biochem, TR-06100 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Schopfer, Lawrence M.</creatorName>
      <givenName>Lawrence M.</givenName>
      <familyName>Schopfer</familyName>
      <affiliation>Univ Nebraska Med Ctr, Eppley Inst, Omaha, NE 68198 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Lockridge, Oksana</creatorName>
      <givenName>Oksana</givenName>
      <familyName>Lockridge</familyName>
      <affiliation>Univ Nebraska Med Ctr, Eppley Inst, Omaha, NE 68198 USA</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Rabbit Antidiethoxyphosphotyrosine Antibody, Made By Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins In Cultured Cells And Immunopurifies Modified Peptides For Mass Spectrometry</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/239402</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1021/acs.jproteome.1c00383</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Chronic low-dose exposure to organophosphorus pesticides is associated with the risk of neurodegenerative disease. The mechanism of neurotoxicity is independent of acetylcholinesterase inhibition. Adducts on tyrosine, lysine, threonine, and serine can occur after exposure to organophosphorus pesticides, the most stable being adducts on tyrosine. Rabbit monoclonal 1C6 to diethoxyphosphate-modified tyrosine (depY) was created by single B cell cloning. The amino acid sequence and binding constant (K-d 3.2 x 10(-8) M) were determined. Cultured human neuroblastoma SH-SY5Y and mouse neuroblastoma N2a cells incubated with a subcytotoxic dose of 10 mu M chlorpyrifos oxon contained depYmodified proteins detected by monoclonal 1C6 on Western blots. depYlabeled peptides from tryptic digests of cell lysates were immunopurified by binding to immobilized 1C6. Peptides released with 50% acetonitrile and 1% formic acid were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on an Orbitrap Fusion Lumos mass spectrometer. Protein Prospector database searches identified 51 peptides modified on tyrosine by diethoxyphosphate in SH-SY5Y cell lysate and 73 diethoxyphosphate-modified peptides in N2a cell lysate. Adducts appeared most frequently on the cytoskeleton proteins tubulin, actin, and vimentin. It was concluded that rabbit monoclonal 1C6 can be useful for studies that aim to understand the mechanism of neurotoxicity resulting from low-dose exposure to organophosphorus pesticides.</description>
  </descriptions>
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