1 Ocak 2022 Dergi makalesi Açık Erişim

Karaaslan, Cagatay; Karaguzel, Dilara; Sarac, Basak Ezgi; Sucularli, Ceren; Bilgic, Hayriye Akel; Kalayci, Omer

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/238728</identifier>
  <creators>
    <creator>
      <creatorName>Karaaslan, Cagatay</creatorName>
      <givenName>Cagatay</givenName>
      <familyName>Karaaslan</familyName>
      <affiliation>Hacettepe Univ, Fac Sci, Mol Biol Sect, Dept Biol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Karaguzel, Dilara</creatorName>
      <givenName>Dilara</givenName>
      <familyName>Karaguzel</familyName>
      <affiliation>Hacettepe Univ, Fac Sci, Mol Biol Sect, Dept Biol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sarac, Basak Ezgi</creatorName>
      <givenName>Basak Ezgi</givenName>
      <familyName>Sarac</familyName>
      <affiliation>Hacettepe Univ, Fac Sci, Mol Biol Sect, Dept Biol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sucularli, Ceren</creatorName>
      <givenName>Ceren</givenName>
      <familyName>Sucularli</familyName>
      <affiliation>Hacettepe Univ, Inst Hlth Sci, Dept Bioinformat, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Bilgic, Hayriye Akel</creatorName>
      <givenName>Hayriye Akel</givenName>
      <familyName>Bilgic</familyName>
      <affiliation>Hacettepe Univ, Fac Sci, Mol Biol Sect, Dept Biol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kalayci, Omer</creatorName>
      <givenName>Omer</givenName>
      <familyName>Kalayci</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Pediat Allergy, Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>The Expression Profile Of Protease Inhibitors In The Airway Epithelial Cells After Allergen (Der P 1) Stimulation</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2022</publicationYear>
  <dates>
    <date dateType="Issued">2022-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/238728</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1159/000518170</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Background: Airway epithelial cells are constantly exposed to intracellular and extracellular proteases that play a pivotal role in several airway diseases. Dermatophagoides pteronyssinus (Der p) 1 derived from house dust mite has protease activity that causes epithelial barrier defect and inflammatory response. Protease inhibitors released against proteases are involved in the maintenance of homeostasis. A disruption of the balance between proteases and protease inhibitors can lead to distortion of the cellular structures and cellular activities and thus culminate in disease processes. Although the effects of Der p 1 allergen on epithelial barrier integrity and inflammatory response are well-established, its contribution to protease inhibitor production is highly limited. Objective: This study aimed to determine the profile of the protease inhibitor response to Der p 1 allergen in human airway epithelial cells, A549 and BEAS-2B. Methods: Differentiated cells by the air-liquid interface were exposed to Der p 1 with or without Th2 type cytokines (IL-4 and IL-13). Gene expression of protease inhibitors was determined by qPCR at 2 different time points. Results: We found that the effect of allergen exposure on the protease inhibitor profile can vary depending on the antigen concentration, treatment duration, and the presence or absence of type 2 cytokines. Gene expressions of serine protease inhibitor (SERPIN)B3 and SERPINB4 were increased following Th2 cytokine stimulation in both cell types at both time points, whereas SERPINB2 and TFPI-2 expressions were induced by 24-h Der p 1 stimulation in both cells. Conclusions: Our study suggests that Der p 1 exposure of the airway epithelium may have consequences related to its protease activity in the presence as well as in the absence of Th2 cytokines in the microenvironment.</description>
  </descriptions>
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