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Effect of increasing dietary protein with constant lysine: methionine ratio on production and omasal flow of nonammonia nitrogen in lactating dairy cows

Gonzalez Ronquillo, M.; Faciola, A. P.; Nursoy, H.; Broderick, G. A.


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/238636</identifier>
  <creators>
    <creator>
      <creatorName>Gonzalez Ronquillo, M.</creatorName>
      <givenName>M.</givenName>
      <familyName>Gonzalez Ronquillo</familyName>
      <affiliation>Univ Autonoma Estado Mexico, Fac Med Vet &amp; Zootecnia, Inst Literario 100, Toluca 50000, Mexico</affiliation>
    </creator>
    <creator>
      <creatorName>Faciola, A. P.</creatorName>
      <givenName>A. P.</givenName>
      <familyName>Faciola</familyName>
      <affiliation>Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Nursoy, H.</creatorName>
      <givenName>H.</givenName>
      <familyName>Nursoy</familyName>
      <affiliation>Bingol Univ, Vet Fac, Dept Anim Nutr, TR-12000 Bingol, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Broderick, G. A.</creatorName>
      <givenName>G. A.</givenName>
      <familyName>Broderick</familyName>
    </creator>
  </creators>
  <titles>
    <title>Effect Of Increasing Dietary Protein With Constant Lysine: Methionine Ratio On Production And Omasal Flow Of Nonammonia Nitrogen In Lactating Dairy Cows</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/238636</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3168/jds.2020-19541</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Eight lactating cows were fed 4 diets in which dietary crude protein (CP) was increased in steps of approximately 2 percentage units from 11 to 17% of DM by replacing high-moisture corn with soybean meal supplemented with rumen-protected Met to maintain a Lys: Met ratio of 3:1 in metabolizable protein. Trial design was a replicated 4 x 4 Latin square; experimental periods lasted 28 d, with data and sample collection being performed during wk 3 and 4 of each period. Digesta samples were collected from the rumen as well as the omasum to measure metabolite concentrations and ruminal outflow of N fractions using infusion of N-15-enriched ammonia to quantify microbial nonammonia N (NAN) and nonmicrobial NAN. Data were analyzed using the MIXED procedure of SAS (SAS Institute Inc.). There were linear increases in the yields of milk and true protein and concentration of milk urea N, and a linear decrease in N efficiency, with increasing dietary CP. Apparent ruminal and total-tract N digestibility increased linearly with increasing dietary CP, but estimated true total-tract N digestibility was not affected. Apparent digestibility of the other macronutrients was not influenced by diet. Ruminal ammonia, total AA and peptides, and branched-chain VFA also increased linearly with dietary CP. The N-15 enrichment of liquidand particle-associated microbes linearly declined with increasing dietary CP due to decreasing 15N enrichment of the ammonia pool. Although no effect of dietary CP on nonmicrobial NAN flow was detected, total NAN flow increased linearly from 525 g/d at 11% CP to 637 g/d at 17% CP due to the linear increase in microbial NAN flow from 406 g/d at 11% CP to 482 g/d at 17% CP. Under the conditions of this study, when dietary CP was increased by adding soybean meal supplemented with rumen-protected Met, improved milk and protein yields were driven not by RUP supply but by increased ruminal outflow of microbial protein.</description>
  </descriptions>
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