1 Ocak 2021 Dergi makalesi Açık Erişim

Pekozer, Gorke Gurel; Akar, Nergis Abay; Cumbul, Alev; Beyzadeoglu, Tahsin; Kose, Gamze Torun

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/237380</identifier>
  <creators>
    <creator>
      <creatorName>Pekozer, Gorke Gurel</creatorName>
      <givenName>Gorke Gurel</givenName>
      <familyName>Pekozer</familyName>
      <affiliation>Yildiz Tech Univ, Fac Elect &amp; Elect Engn, Biomed Engn Dept, TR-34220 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Akar, Nergis Abay</creatorName>
      <givenName>Nergis Abay</givenName>
      <familyName>Akar</familyName>
      <affiliation>Yeditepe Univ, Genet &amp; Bioengn Dept, Fac Engn, TR-34755 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Cumbul, Alev</creatorName>
      <givenName>Alev</givenName>
      <familyName>Cumbul</familyName>
      <affiliation>Yeditepe Univ, Fac Med, Histol &amp; Embryol Dept, TR-34755 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Beyzadeoglu, Tahsin</creatorName>
      <givenName>Tahsin</givenName>
      <familyName>Beyzadeoglu</familyName>
      <affiliation>Halic Univ, Fac Hlth Sci, Orthopaed &amp; Traumatol, Beyzadeoglu Clin, TR-34738 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kose, Gamze Torun</creatorName>
      <givenName>Gamze Torun</givenName>
      <familyName>Kose</familyName>
      <affiliation>Yeditepe Univ, Genet &amp; Bioengn Dept, Fac Engn, TR-34755 Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Investigation Of Vasculogenesis Inducing Biphasic Scaffolds For Bone Tissue Engineering</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/237380</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1021/acsbiomaterials.0c01071</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Vascularization is the main obstacle for the bone tissue engineering strategies since the defect size is generally large. Incorporation of angiogenic factors is one of the strategies employed in order to accelerate vascularization and improve bone healing. In this study, a biphasic scaffold consisting of fibrous poly(lactide-co-glycolide) (PLGA) and poly(lactide-co-glycolide)block- poly( ethylene glycol)-block-poly(lactide-co-glycolide) (PLGA-PEG-PLGA) hydrogel loaded with vascular endothelial growth factor-A (VEGF) inducer, GS4012, was constructed. Mesenchymal stem cells isolated from rat bone marrow (rBMSCs) were used for differentiation into osteogenic cells, and endothelial cells isolated from rat peripheral blood (rPBECs) were used to test the in vitro endothelial cell recruitment. The biphasic scaffold was tested for cell proliferation, ALP expression, VEGF induction, expression of osteogenic genes by rBMSCs, and recruitment of rPBECs in vitro and for improved bone healing and vascularization in vivo on critical size rat cranial defects. Endothelial migration through porous insert and VEGF induction were obtained in vitro in response to GS4012 as well as the upregulation of ALP, Runx2, Col I, and OC gene expressions. The biphasic scaffold was also shown to be effective in improving endothelial cell recruitment, vascularization, and bone healing in vivo. Thus, the proposed design has a great potential for the healing of critical size bone defect in tissue engineering studies according to both in vitro and in vivo investigations.</description>
  </descriptions>
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