1 Ocak 2021 Dergi makalesi Açık Erişim

Keskin, Ezgi Gul; Huang, Jialiang; Terzi Cizmecioglu, Nihal

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/234868</identifier>
  <creators>
    <creator>
      <creatorName>Keskin, Ezgi Gul</creatorName>
      <givenName>Ezgi Gul</givenName>
      <familyName>Keskin</familyName>
      <affiliation>Middle East Tech Univ, Fac Arts &amp; Sci, Dept Biol Sci, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Huang, Jialiang</creatorName>
      <givenName>Jialiang</givenName>
      <familyName>Huang</familyName>
      <affiliation>Xiamen Univ, Innovat Ctr Cell Signaling Network, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen, Fujian, Peoples R China</affiliation>
    </creator>
    <creator>
      <creatorName>Terzi Cizmecioglu, Nihal</creatorName>
      <givenName>Nihal</givenName>
      <familyName>Terzi Cizmecioglu</familyName>
      <affiliation>Middle East Tech Univ, Fac Arts &amp; Sci, Dept Biol Sci, Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Arid4B Physically Interacts With Tfap2C In Mouse Embryonic Stem Cells</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/234868</alternateIdentifier>
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  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3906/biy-2010-67</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Precise regulation of gene expression is required for embryonic stem cell (ESC) differentiation. Transcription factor (TF) networks coordinate the balance of pluripotency and differentiation in response to extracellular and intracellular signals. Chromatin factors work alongside TFs to achieve timely regulation of gene expression for differentiation process. Our previous studies showed that a member of the Sin3a corepressor complex, Arid4b, is critical for proper mouse ESC differentiation into mesoderm and endoderm. We found elevated histone 3 lysine 27 acetylation (H3K27Ac) in a subset of genomic loci in meso/endoderm directed arid4b. cells, coincident with their derepression. We reasoned that Sin3a complex may be required for the suppression of these genes during differentiation. To identify TFs that might cooperate with Arid4b for this function, we found consensus TF binding sequences enriched in H3K27Ac elevated regions in arid4b. cells. Of these candidate TFs, we validated expression of Bach1, Ddit3, Prrx2, Znf354c and Tfap2c in mESCs. We then demonstrated a physical interaction between Arid4b and Tfap2c in mESCs using endogenous coimmunoprecipitation and proximity ligation assay experiments. Our results point to a role of Arid4b in the Sin3a complex in repression of a subset of Tfap2cregulated genes during meso/endoderm differentiation.</description>
  </descriptions>
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