Dergi makalesi Açık Erişim
Atabey, Tugba; Acar, Tayfun; Derman, Serap; Ordu, Emel; Erdemir, Aysegul; Tasli, Pakize Neslihan; Gur, Gunseli Kurt; Sahin, Fikrettin; Gulluce, Medine; Arasoglu, Tulin
<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
<leader>00000nam##2200000uu#4500</leader>
<datafield tag="245" ind1=" " ind2=" ">
<subfield code="a">In Vitro Evaluation of Immunogenicity of Recombinant OMP25 Protein Obtained from Endemic Brucella abortus Biovar 3 as Vaccine Candidate Molecule Against Animal Brucellosis</subfield>
</datafield>
<datafield tag="909" ind1="C" ind2="4">
<subfield code="p">PROTEIN AND PEPTIDE LETTERS</subfield>
<subfield code="v">28</subfield>
<subfield code="n">10</subfield>
<subfield code="c">1138-1147</subfield>
</datafield>
<controlfield tag="001">234116</controlfield>
<datafield tag="980" ind1=" " ind2=" ">
<subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield>
</datafield>
<datafield tag="520" ind1=" " ind2=" ">
<subfield code="a">Background: Brucellosis is a zoonotic disease that causes serious economic losses due to factors, such as miscarriages and decreased milk yield in animals. Existing live vaccines have some disadvantages, so effective vaccines need to be developed with new technological approaches. Objective: The primary objectives of this study were the expression and purification of recombinant Omp25 fusion protein from B. abortus, and the evaluation of the effect of the Omp25 protein on cell viability and inflammatory response. Methods: The omp25 gene region was amplified by a polymerase chain reaction and cloned into a Pet102/D-TOPO expression vector. The protein expression was carried out using the prokaryotic expression system. The recombinant Omp25 protein was purified with affinity chromatography followed by GPC (Gel Permeation Chromatography). The MTS assay and cytokine-release measurements were carried out to evaluate cell viability and inflammatory response, respectively. Results: It was determined that doses of the recombinant Omp25 protein doses greater than 0.1 mu g/mL are toxic to RAW cells. Doses of 1 mu g/mL and lower significantly increased inflammation due to nitric oxide (NO) levels. ELISA results showed that IFN-gamma was produced in stimulated RAW 264.7 cells at a dose that did not affect the viability (0.05 mu g/mL). However, IL-12, which is known to have a dual role in the activation of macrophages, did not show a statistically significant difference at the same dose. Conclusion: Studies on cell viability and Th1-related cytokine release suggest Omp25 protein to be a promising candidate molecule for vaccine development.</subfield>
</datafield>
<datafield tag="650" ind1="1" ind2="7">
<subfield code="2">opendefinition.org</subfield>
<subfield code="a">cc-by</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Chem & Met Engn, Bioengn Dept, Istanbul, Turkey</subfield>
<subfield code="a">Acar, Tayfun</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Chem & Met Engn, Bioengn Dept, Istanbul, Turkey</subfield>
<subfield code="a">Derman, Serap</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Arts & Sci, Mol Biol & Genet Dept, Istanbul, Turkey</subfield>
<subfield code="a">Ordu, Emel</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Arts & Sci, Mol Biol & Genet Dept, Istanbul, Turkey</subfield>
<subfield code="a">Erdemir, Aysegul</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, Istanbul, Turkey</subfield>
<subfield code="a">Tasli, Pakize Neslihan</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Arts & Sci, Mol Biol & Genet Dept, Istanbul, Turkey</subfield>
<subfield code="a">Gur, Gunseli Kurt</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, Istanbul, Turkey</subfield>
<subfield code="a">Sahin, Fikrettin</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Ataturk Univ, Fac Sci, Biol Dept, Erzurum, Turkey</subfield>
<subfield code="a">Gulluce, Medine</subfield>
</datafield>
<datafield tag="700" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Arts & Sci, Mol Biol & Genet Dept, Istanbul, Turkey</subfield>
<subfield code="a">Arasoglu, Tulin</subfield>
</datafield>
<datafield tag="980" ind1=" " ind2=" ">
<subfield code="b">article</subfield>
<subfield code="a">publication</subfield>
</datafield>
<datafield tag="542" ind1=" " ind2=" ">
<subfield code="l">open</subfield>
</datafield>
<datafield tag="100" ind1=" " ind2=" ">
<subfield code="u">Yildiz Tech Univ, Fac Arts & Sci, Mol Biol & Genet Dept, Istanbul, Turkey</subfield>
<subfield code="a">Atabey, Tugba</subfield>
</datafield>
<datafield tag="260" ind1=" " ind2=" ">
<subfield code="c">2021-01-01</subfield>
</datafield>
<controlfield tag="005">20221007085437.0</controlfield>
<datafield tag="909" ind1="C" ind2="O">
<subfield code="o">oai:aperta.ulakbim.gov.tr:234116</subfield>
<subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield>
</datafield>
<datafield tag="856" ind1="4" ind2=" ">
<subfield code="z">md5:116b7d86e259d0e76329e7975a97ab15</subfield>
<subfield code="s">343</subfield>
<subfield code="u">https://aperta.ulakbim.gov.trrecord/234116/files/bib-055fe544-a239-4548-bf82-4edd60d0cee1.txt</subfield>
</datafield>
<datafield tag="540" ind1=" " ind2=" ">
<subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield>
<subfield code="a">Creative Commons Attribution</subfield>
</datafield>
<datafield tag="024" ind1=" " ind2=" ">
<subfield code="a">10.2174/0929866528666210615104334</subfield>
<subfield code="2">doi</subfield>
</datafield>
</record>
| Görüntülenme | 21 |
| İndirme | 11 |
| Veri hacmi | 3.8 kB |
| Tekil görüntülenme | 19 |
| Tekil indirme | 11 |