Dergi makalesi Açık Erişim

The effects of L-carnitine levels on population growth of rotifer Brachionus plicatilis fed with Chlorella vulgaris

2021    Ozdogan, H. B. E.; Savas, S.

In this study, S-type rotifer Brachionus plicatilis were enriched with 10 levels (0, 0.001, 0.01, 0.1, 1, 10, 100, 500, 1000 and 1500 mg L-carnitine per L) of L-carnitine supplements dissolved in Chlorella vulgaris culture medium in a 10 mL individual culture trial for 7 days. The initial rotifer density was 1 individual mL(-1). The trials were conducted to 200x0(6) cell mL(-1) at food density at 25 +/- 1 degrees C under an axenic condition in the laboratory. L-carnitine enrichment has shown considerable influence on the population growth of rotifer Brachionus plicatilis. As a result, the maximum number of individuals was determined as 1560 +/- 10.00 rot./mL in the group enriched with 100 mg/L L-carnitine supplement and the lowest number of individuals was determined 376.67 +/- 25.17 rot./mL in the group enriched with 1500 mg/L L-carnitine addition (p<0.05). The maximum growth rate was found to be 0.63 +/- 0.02 rot/ day in the group enriched with 100 mg/L L-carnitine addition (p<0.05). The maximum doubling time was 1.31 +/- 0.02 rot./ day for the group enriched with 1000 mg/L L-carnitine (p<0.05). The results suggested that L-carnitine could be a positive factor to enhance reproduction and population growth on enriched Brachionus plicatilis under the optimum concentration.