1 Ocak 2013 Dergi makalesi Açık Erişim

Akca, Hakan; Demiray, Aydin; Yaren, Arzu; Bir, Ferda; Koseler, Aylin; Iwakawa, Reika; Bagci, Gulseren; Yokota, Jun

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{
  "@context": "https://schema.org/", 
  "@id": 13591, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Med Biol, Denizli, Turkey", 
      "name": "Akca, Hakan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Med Biol, Denizli, Turkey", 
      "name": "Demiray, Aydin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Med Oncol, Denizli, Turkey", 
      "name": "Yaren, Arzu"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Pathol, Denizli, Turkey", 
      "name": "Bir, Ferda"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Biophys, Denizli, Turkey", 
      "name": "Koseler, Aylin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Canc Ctr, Res Inst, Div Multistep Carcinogenesis, Tokyo 104, Japan", 
      "name": "Iwakawa, Reika"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Med Biol, Denizli, Turkey", 
      "name": "Bagci, Gulseren"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Canc Ctr, Res Inst, Div Multistep Carcinogenesis, Tokyo 104, Japan", 
      "name": "Yokota, Jun"
    }
  ], 
  "datePublished": "2013-01-01", 
  "description": "Mutations in the EGFR gene are critical determinants of treatment with EGFR tyrosine kinase inhibitors (TKIs) for non-small cell lung cancer (NSCLC) patients. DNA isolation from tumor samples usually requires surgery; therefore, we wanted to isolate DNA from circulating tumor cells by using the serum of NSCLC patients. This protocol was recently published. DNA was isolated from the serum of 52 Turkish NSCLC patients and their EGFR mutation status was examined by pyrosequencing. EGFR mutations were detected in 25 of the 52 patients (48.1%): 17 patients with delE746-A750, 2 with delE747-A750insP, and 6 with L858R. All mutations detected by pyrosequencing were confirmed by dideoxy sequencing, and the presence of the same mutations in the tumors was verified by using paraffin embedded tissues of all the patients. Mutations were detected more frequently in adenocarcinomas (24 of 36, 66.7%) than in squamous cell carcinomas (1 of 16, 6.3%) (P < 0.001). These results confirm the utility of serum DNA and pyrosequencing for the detection of EGFR mutations in patients with advanced NSCLC.", 
  "headline": "Utility of serum DNA and pyrosequencing for the detection of EGFR mutations in non-small cell lung cancer", 
  "identifier": 13591, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Utility of serum DNA and pyrosequencing for the detection of EGFR mutations in non-small cell lung cancer", 
  "url": "https://aperta.ulakbim.gov.tr/record/13591"
}