1 Ocak 2021 Dergi makalesi Açık Erişim

Ozcan, Emrah; Seven, Merve; Sirin, Burcu; Cakir, Tunahan; Nikerel, Emrah; Teusink, Bas; Toksoy Oner, Ebru

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{
  "DOI": "10.1002/bit.27565", 
  "abstract": "In this study, we have investigated the cheese starter culture as a microbial community through a question: can the metabolic behaviour of a co-culture be explained by the characterized individual organism that constituted the co-culture? To address this question, the dairy-origin lactic acid bacteriaLactococcus lactissubsp.cremoris,Lactococcus lactissubsp.lactis,Streptococcus thermophilus andLeuconostoc mesenteroides, commonly used in cheese starter cultures, were grown in pure and four different co-cultures. We used a dynamic metabolic modelling approach based on the integration of the genome-scale metabolic networks of the involved organisms to simulate the co-cultures. The strain-specific kinetic parameters of dynamic models were estimated using the pure culture experiments and they were subsequently applied to co-culture models. Biomass, carbon source, lactic acid and most of the amino acid concentration profiles simulated by the co-culture models fit closely to the experimental results and the co-culture models explained the mechanisms behind the dynamic microbial abundance. We then applied the co-culture models to estimate further information on the co-cultures that could not be obtained by the experimental method used. This includes estimation of the profile of various metabolites in the co-culture medium such as flavour compounds produced and the individual organism level metabolic exchange flux profiles, which revealed the potential metabolic interactions between organisms in the co-cultures.", 
  "author": [
    {
      "family": "Ozcan", 
      "given": " Emrah"
    }, 
    {
      "family": "Seven", 
      "given": " Merve"
    }, 
    {
      "family": "Sirin", 
      "given": " Burcu"
    }, 
    {
      "family": "Cakir", 
      "given": " Tunahan"
    }, 
    {
      "family": "Nikerel", 
      "given": " Emrah"
    }, 
    {
      "family": "Teusink", 
      "given": " Bas"
    }, 
    {
      "family": "Toksoy Oner", 
      "given": " Ebru"
    }
  ], 
  "container_title": "BIOTECHNOLOGY AND BIOENGINEERING", 
  "id": "10867", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "223-237", 
  "title": "Dynamic co-culture metabolic models reveal the fermentation dynamics, metabolic capacities and interplays of cheese starter cultures", 
  "type": "article-journal", 
  "volume": "118"
}