1 Ocak 2020 Dergi makalesi Açık Erişim

Degitz, Ilayda Acaroglu; Gazioglu, Berk Hakki; Aksu, Mehmet Burak; Malta, Seyda; Sezer, Ali Demir; Eren, Tarik

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/10825</identifier>
  <creators>
    <creator>
      <creatorName>Degitz, Ilayda Acaroglu</creatorName>
      <givenName>Ilayda Acaroglu</givenName>
      <familyName>Degitz</familyName>
      <affiliation>Yeditepe Univ, Dept Chem Engn, Kayisdagi Cd 326A, TR-34755 Atasehir Maltepe Istanbu, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Gazioglu, Berk Hakki</creatorName>
      <givenName>Berk Hakki</givenName>
      <familyName>Gazioglu</familyName>
      <affiliation>Yeditepe Univ, Dept Chem Engn, Kayisdagi Cd 326A, TR-34755 Atasehir Maltepe Istanbu, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Aksu, Mehmet Burak</creatorName>
      <givenName>Mehmet Burak</givenName>
      <familyName>Aksu</familyName>
      <affiliation>Marmara Univ, Dept Med Microbiol, Maltepe Basibuyuk Yolu Sok 9-2, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Malta, Seyda</creatorName>
      <givenName>Seyda</givenName>
      <familyName>Malta</familyName>
      <affiliation>Yeditepe Univ, Dept Chem Engn, Kayisdagi Cd 326A, TR-34755 Atasehir Maltepe Istanbu, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sezer, Ali Demir</creatorName>
      <givenName>Ali Demir</givenName>
      <familyName>Sezer</familyName>
      <affiliation>Marmara Univ, Dept Pharm, Tibbbiye Cad 49, TR-34668 Uskudar Instanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Eren, Tarik</creatorName>
      <givenName>Tarik</givenName>
      <familyName>Eren</familyName>
      <affiliation>Yildiz Tech Univ, Dept Chem, Davutpasa Kampusu, TR-34220 Esenler Instanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Antibacterial And Hemolytic Activity Of Cationic Polymer-Vancomycin Conjugates</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2020</publicationYear>
  <dates>
    <date dateType="Issued">2020-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/10825</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.eurpolymj.2020.110084</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">In this study, a Gram positive bacteria effective anti-biotic, vancomycin (VAN), was conjugated with antimicrobial cationic polymers (ACPs) that were found to be active against both Gram positive and Gram negative bacteria previously, and the antibacterial activity of newly synthesized VAN-PEG-ACP conjugates were investigated. VAN was first PEGylated using Michael Addition method, then the ACPs, which were synthesized using ring-opening metathesis polymerization (ROMP), were bound covalently to the VAN-PEG structure using cross metathesis pathways and thus cationic polymer-vancomycin conjugates (VAN-PEG-ACPs) were obtained. The antimicrobial activity of the conjugates were investigated against Gram negative strain E. coli and Gram positive strains S. aureus and E. feacalis. Con_1_10k_1, Con_2a_3k_1, and Con_2b_3k_1 were found to be active towards S. aureus (MIC = 64 mu g/ml, 32 mu g/mL, and 32 mu g/mL, respectively) and Con_2a_3k_1, and Con_2b_3k_l were found to be active also towards E. feacalis (MIC = 16 mu g/ml and 32 mu g/mL, respectively), however they did not show activity towards E. coli. All the conjugates were found to be non-hemolytic towards red blood cells (HC50 = &amp;gt; 1024 mu g/mL). MTS assay results also showed that the conjugates did not lyse viable HUVEC cells within 72 h. The membrane deformation of S. aureus was monitored using scanning electron microscopy (SEM) techniques in the presence of the polymers and the conjugates.</description>
  </descriptions>
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