Published January 1, 2019
| Version v1
Journal article
Open
3D bio-printing of levan/polycaprolactone/gelatin blends for bone tissue engineering: Characterization of the cellular behavior
Creators
- 1. Marmara Univ, Fac Technol, Dept Text Engn, Goztepe Campus, TR-34722 Istanbul, Turkey
- 2. Istanbul Arel Univ, ArelPOTKAM Polymer Technol & Composite A&R Ctr, Istanbul, Turkey
- 3. Marmara Univ, Fac Engn, Dept Chem Engn, TR-34722 Istanbul, Turkey
Description
Poly(epsilon-caprolactone) (PCL), gelatin (GT) and different concentrations of low molecular weight Halomonas levan (HLh) were combined and examined to develop physical networks serving as tissue scaffolds to promote cell adhesion for biocompatibility. Three-dimensional bioprinting technique (3D bioprinting) was employed during manufacturing the test samples and their comprehensive characterization was performed to investigate the physicochemical properties and biocompatibility. Physical properties of the printing materials such as viscosity, surface tension, and density were measured to determine optimal parameters for 3D bioprinting. The scanning electron microscope (SEM) was used to observe the morphological structure of scaffolds. Fourier-Transform Infrared Spectroscopy (FT-IR) and differential scanning calorimetry (DSC) were used to identify the interactions between the components. In-vitro cell culture assays using standard human osteoblast (Hob) cells showed increased biocompatibility of the printing materials with increasing HLh content. Thus, the formulations including the HLh are expected to be a good candidate for the production of 3D printed materials.
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