Phenolic acid contents of Salvia poculata Nab by LC-MS/MS

Phenolic acids, biological aromatic secondary plant metabolites, are widely distributed throughout the plant kingdom. Due to the biological importance of phenolic acids as secondary metabolites, development of analytical methods for the determination of phenolic acids crucial. In the present study, a simple, rapid LC-MS/MS method was developed and validated for phenolic acid determination in methanol extract of Salvia poculate Nab from Turkey. The relative standard deviations (RSD) were found to be 3.01, 5.99, 3.21, 2.07 and 5.59 % for phenolic acids caffeic acid, fumaric acid, p-coumaric acid, syringic acid and t-ferulic acid respectively. The correlation coefficient was greater than 0.99 for each analytes in the calibration range. The uncertainty of the measurements for phenolic compounds were 7.31%, 9.27%, 7.34, 6.78% and 9.11% respectively.