Published January 1, 2017
| Version v1
Journal article
Open
A smartphone based surface plasmon resonance imaging (SPRi) platform for on-site biodetection
Creators
- 1. Bilkent Univ, Inst Mat Sci & Nanotechnol, UNAM, TR-06800 Ankara, Turkey
- 2. Sci & Technol Res Council Turkey TUBITAK, UEKAE BILGEM, TR-41470 Gebze, Kocaeli, Turkey
- 3. Middle East Tech Univ, Dept Comp Engn, TR-06800 Ankara, Turkey
- 4. Stanford Sch Med, Dept Radiol, Canary Ctr Stanford Canc Early Detect, Palo Alto, CA 94304 USA
Description
We demonstrate a surface plasmon resonance imaging platform integrated with a smartphone to be used in the field with high-throughput biodetection. Inexpensive and disposable SPR substrates are produced by metal coating of commercial Blu-ray discs. A compact imaging apparatus is fabricated using a 3D printer which allows taking SPR measurements from more than 20.000 individual pixels. Real-time bulk refractive index change measurements yield noise equivalent refractive index changes as low as 4.12 x 10(-5) RIU which is comparable with the detection performance of commercial instruments. As a demonstration of a biological assay, we have shown capture of mouse IgG antibodies by immobilized layer of rabbit anti-mouse (RAM) IgG antibody with nanomolar level limit of detection. Our approach in miniaturization of SPR biosensing in a cost-effective manner could enable realization of portable SPR measurement systems and kits for point-of-care applications. (C) 2016 Published by Elsevier B.V.
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