Published January 1, 2001
| Version v1
Journal article
Open
Neutral fluorescence probe with strong ratiometric response to surface charge of phospholipid membranes
Creators
Description
We report on dramatic differences in fluorescence spectra of 4'-dimethylamino-3-hydroxyflavone (probe F) studied in phospholipid membranes of different charge (phosphatidyl glycerol, phosphatidylcholine (PC), their mixture and the mixture of PC with a cationic lipid). The effect consists in variations of relative intensities at two well-separated band maxima at 520 and 570 nm belonging to normal (N*) and tautomer (T*) excited states of flavone chromophore. Based on these studies we propose a new approach to measure electrostatic potential at the surface layer of phospholipid membranes, which is based on potential-dependent changes of bilayer hydration and involves very sensitive and convenient ratiometric measurements in fluorescence emission. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Files
bib-a9ed2da9-87b0-4bcf-bc2c-dfe9353d1990.txt
Files
(199 Bytes)
| Name | Size | Download all |
|---|---|---|
|
md5:a976961090fc81394f04171924baf7a8
|
199 Bytes | Preview Download |