Published January 1, 2020 | Version v1
Journal article Open

A CASE-STUDY OF THE MOLECULAR DIAGNOSIS OF ECHINOCOCCUS MULTILOCULARIS IN WILD BOAR WITH COMMENTS ON ITS PUBLIC HEALTH SIGNIFICANCE IN TURKEY

  • 1. Bingol Univ, Fac Vet Med, Dept Parasitol, TR-12000 Bingol, Turkey
  • 2. Firat Univ, Fac Vet Med, Dept Parasitol, TR-23119 Elazig, Turkey
  • 3. COMSATS Univ Islamabad CUI, Dept Biosci, Pk Rd, Islamabad, Pakistan

Description

Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.

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