Yayınlanmış 1 Ocak 2010
| Sürüm v1
Dergi makalesi
Açık
Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles
Oluşturanlar
- 1. Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey
- 2. Natl Metrol Inst, TUBITAK UME, TR-41470 Gebze, Turkey
- 3. Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey
- 4. Royal Inst Technol KTH, Dept Funct Mat, S-16440 Stockholm, Sweden
Açıklama
In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.
Dosyalar
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Dosyalar
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