Published January 1, 2010 | Version v1
Journal article Open

An EST-SSR marker linked with yellow rust resistance in wheat

Creators

  • 1. Marmara Univ, Dept Biol, Fac Sci & Letters, TR-34722 Istanbul, Turkey
  • 2. TUBITAK, MRC, Genet Engn & Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey
  • 3. Gebze Inst Technol, Fac Sci, Dept Biol, TR-41470 Gebze, Kocaeli, Turkey
  • 4. Field Crop Res Inst, TR-06042 Ankara, Turkey
  • 5. Anatolian Agr Res Inst, TR-26001 Eskisehir, Turkey

Description

Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F-2 seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F-2 hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance.

Files

bib-5a777156-2832-4405-ab6d-cf3e966707f3.txt

Files (241 Bytes)

Name Size Download all
md5:cb3b260b9bc65a34015434f8e9fb530f
241 Bytes Preview Download