Ovarian antiproliferative activity directed isolation of triterpenoids from fruits of Eucalyptus camaldulensis Dehnh

Bioassay-guided fractionation of a methanol extract of the fruits of Eucalyptus camaldulensis Dehnh. with moderate antiproliferative activity afforded the new triterpene, 3 beta-acetoxy-urs-11,13(18)-dien-28-oic acid (1) along with the known triterpenoids 3 beta-hydroxy-urs-11-en-28,13 beta-olide (2), 3 beta-acetoxy-urs-11-en-28,13 beta-olide (3), 3-acetylbetulinic acid (4), oleanolic acid (5), ursolic acid (6), beta-amyrin acetate (7), beta-sitosterol (8) and sitosterol 3-O-beta-D-glucopyranoside (9). Their structures were established on the basis of analysis of their (1)H and (13)C NMR, APT, gHSQC, gHMBC, UV, IR and mass spectral data. The tested triterpenoids (1-7) exhibited weak-moderate antiproliferative activity against the A2780 human ovarian cancer cell line. (C) 2011 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.