Published January 1, 2011
| Version v1
Journal article
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Polysulfone/Pyrene Membranes: A New Microwell Assay Platform for Bioapplications
Creators
- 1. Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey
- 2. Istanbul Tech Univ, Dept Chem, TR-34469 Istanbul, Turkey
- 3. Adnan Menderes Univ, Sch Med, Dept Infect Dis & Clin Microbiol, TR-09100 Aydin, Turkey
- 4. TUBITAK Marmara Res Ctr, Inst Chem, TR-41470 Gebze, Kocaeli, Turkey
Description
The use of PSU-Py prepared by click chemistry as a platform in membrane-bottom microwell plates for oxidase and hydrolase/oxidase-based enzyme assays is studied. For the GOx assay, the postulated fluorescence mechanism is based on the consumption of glucose by dissolved oxygen and GOx in the microwell plates covered with the PSU-Py membrane. For the AG-GOx assay, maltose is used as AG substrate and hydrolyzed to glucose which is then oxidized by the GOx activity. It is shown that the PSU-Py membrane acts as a fluorescence indicator of the enzymatic reactions, and both GOx and AG/GOx enzyme assays are successfully applied for glucose, maltose and acorbose analysis in the range 0.125-2.0 x 10(-3) M glucose, 0.05-0.5 x 10(-3) M maltose, and 0.0125-0.1 mg . mL(-1) acorbose, respectively.
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