Functionalization of Reactive Polymeric Coatings via Diels-Alder Reaction Using Microcontact Printing

The fabrication of polymeric thin films amenable to facile functionalization by reactive mu CP via a DielsAlder reaction is described. Precursor copolymers containing FuMA, PEGMA, and TMSMA are prepared using ATRP. Surface-tethered thin films of these polymers are obtained on oxidized silicon and glass substrates and patterned with maleimide-appended dye molecules by simple mu CP to demonstrate efficient functionalization via DielsAlder reaction. Printing of biotin-based ligands is carried out to demonstrate directed immobilization of the enzyme streptavidin. Due to the thermoreversible nature of the DielsAlder reaction, these surfaces can be used as rewritable platforms. This is demonstrated by sequential writeeraserewrite protocols via mu CP of a maleimide-containing fluorescent dye.