Published January 1, 2015
| Version v1
Journal article
Open
Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)
Creators
- 1. Koc Univ, Sch Med, TR-34450 Istanbul, Turkey
- 2. Istanbul Univ, Fac Med, TR-34093 Istanbul, Turkey
Description
Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line. (C) 2015 The Authors. Published by Elsevier B.V.
Files
bib-98d7f979-957e-4421-92af-c42b25183e05.txt
Files
(253 Bytes)
| Name | Size | Download all |
|---|---|---|
|
md5:c25e48b3f49d3154a31b499536d37d07
|
253 Bytes | Preview Download |