The effects of plant growth regulators and L-phenylalanine on phenolic compounds of sweet basil

The effects of methyl jasmonate (MeJA), spermine (Spm), epibrassinolide (EBL) and L-phenylalanine on sweet basil (Ocimum basilicum L) were studied to determine the amount of phenolic compounds and enzymatic activity of phenylalanine ammonia-lyase (PAL). Total phenolic and total flavonoid contents of sweet basils were determined by a spectrophotometer, and individual phenolic compounds and activity of PAL were analysed by HPLC/UV. The highest total phenolic (6.72 mg GAE/g) and total flavonoid contents (0.92 mg QE/g) obtained from 1.0 mM Spm + MeJA application. Rosmarinic acid (RA) and caffeic acid contents significantly enhanced after the applications but no such differences observed in chicoric acid content or PAL activity. RA was the main phenolic acid in all samples and its concentration varied from 1.04 to 2.70 mg/g FW. As a result the combinations of Spm + MeJA and EBL + MeJA can induce secondary metabolites effectively and those interactions play important role in the production of phytochemicals in plants. (C) 2014 Elsevier Ltd. All rights reserved.