Determination of epinephrine in human serum using a Pt electrode modified by polyaniline-poly(3-methylthiophene)-poly(3,3 '-diaminobenzidine)

A Pt electrode modified by polyaniline-poly(3-methylthiophene)-poly(3,3'-diaminobenzidine) was used for amperometric determination of epinephrine in a solution of NaHSO4/Na2SO4 (pH 2.0). For these studies, potentials between 0.40 and 0.50 V were applied, and the best response was obtained at 0.45 V. The limit of detection, limit of quantification, and the linear dynamic range were 1.23 x 10(-4), 4.10 x 10(-4), and 4.10 x 10(-4) to 100.0 mmol L-1, respectively. These results were compared with determinations using Pt electrodes that were coated and uncoated with homopolymers. To check the accuracy of the developed method and the matrices interference, determination of epinephrine was performed in human serum samples using this modified electrode. The epinephrine concentrations were adjusted to 1.0 and 5.0 mmol L-1, and recovery values were calculated as 100.4% and 96.8%, respectively. It is significant that the determination of epinephrine was carried out at a lower potential (0.45 V) than the oxidation potential of epinephrine (0.65 V) without any matrix effect.