Published January 1, 2015
| Version v1
Journal article
Open
Bioapplications of Polythiophene-g-Polyphenylalanine-Covered Surfaces
Creators
- 1. Istanbul Tech Univ, Fac Sci & Letters, Dept Chem, TR-34469 Istanbul, Turkey
- 2. Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey
- 3. Ege Univ, Grad Sch Nat & Appl Sci, Dept Biotechnol, TR-35100 Izmir, Turkey
- 4. Dicle Univ, Fac Sci, Dept Chem, TR-21280 Diyarbakir, Turkey
- 5. Kinki Univ, Mol Engn Inst, Iizuka, Fukuoka 8208555, Japan
Description
The fabrication of electro and bioactive surfaces by electrochemical deposition of the thiophene-functionalized polyphenylalanine macromonomer (T-g-PPhe) is reported. The resulting conducting graft copolymer, polythiophene-graft-polyphenylalanine (PT-g-PPhe) formed on the indium tin oxide (ITO) glass surface, is characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and fluorescence microscopy. Then, possible uses of PT-g-PPhe as matrices in the sensor design for both electrochemical biosensing and cell adhesion studies are investigated. In the first part, PT-g-PPhe that is deposited on ITO is further functionalized with the arginylglycylaspartic acid peptide via 1-Ethyl-3-(3 dimethylaminopropyl) carbodiimide for the selective cell adhesion. Immunofluorescence staining is performed to detect the difference between adherences of "integrin alpha v beta 3" receptor positive (U87-MG) and negative (HaCaT) cell lines on to the biofunctional surface. In the second part, an electrochemical glucose sensor is constructed by immobilizing glucose oxidase on the surface of PT-g-PPhe, which is deposited on a glassy carbon electrode.
Files
bib-f8fb1fa8-5cc1-41f2-adf6-cd08dca23a93.txt
Files
(283 Bytes)
| Name | Size | Download all |
|---|---|---|
|
md5:fb0e0a2c5b13faa0278c4ade342745b8
|
283 Bytes | Preview Download |