Published January 1, 2015
| Version v1
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Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila
Creators
- 1. Anadolu Univ, Grad Sch Sci, Dept Adv Technol, Biotechnol Program, Eskisehir, Turkey
- 2. Anadolu Univ, Fac Sci, Dept Biol, Eskisehir, Turkey
Description
Background: A superior Green Fluorescent Protein (GFP) mutant, known as superfolder GFP (sfGFP), is more soluble, faster folding, and is the brightest of the known GFP mutants. This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purification in Tetrahymena thermophila.
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