Multiwalled Carbon Nanotubes-Chitosan Modified Single-Use Biosensors for Electrochemical Monitoring of Drug-DNA Interactions

A multiwalled carbon nanotubes (CNT)-chitosan (CHIT) modified pencil graphite electrode (CNT-CHIT/PGE) was developed for the first time herein for electrochemical monitoring of the interaction of an anticancer drug, mitomycin C (MC) and DNA. The characterization of unmodified PGE, CHIT/PGE, CNT/PGE and CHIT-CNT/PGE were performed by scanning electron microscopy and cyclic voltammetry techniques. The oxidation signals of MC and guanine were measured before and after interaction at the surface of CNT-CHIT/PGEs using differential pulse voltammetry. Electrochemical impedance spectroscopy technique was also successfully utilized for monitoring of the interaction process at the surface of CNT-CHIT/PGEs in different interaction times.