Published January 1, 2019
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DNA ligase photocrosslinked cryogenic column based biotinylation kit for viral hybridization and detection
- 1. Anadolu Univ, Chem Dept, Anadolu Teknopk 212, TR-26470 Eskisehir, Turkey
- 2. Kafkas Univ, Bioengn Dept, TR-36200 Kars, Turkey
Description
This study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach "ANADOLUCA" for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5' end as a first. In this process, which does not require ATP ligand, CCHF recognition has been performed using stable and quantum dots (QDs) based avidity nanoparticles cross-linked with streptavidin.
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