Assessment of a new dual effective combo polymer structure for separation of lysozyme from hen egg white

Water-soluble O-carboxymethyl chitosan (O-CMCS) Schiff base granules were prepared, and Cu2+ ions were decorated. Then, dual effective combo cryogel structures were prepared by free radical polymerization using Cu2+-O-CMCS granules as embedding agent. 2-Hydroxyethyl methacrylate and N,N '-methylene-bis-acrylamide were used as monomer and cross-linker pair, respectively. Prepared combo structures were characterized by FTIR, SEM and swelling tests measurements, and investigated for lysozyme adsorption. Maximum lysozyme adsorption was observed in phosphate buffer of pH 8.0 as 103.3 mg/g with initial lysozyme concentration of 3 mg/mL. On the other hand, the most efficient adsorption was carried out at 0.5 mL/min, at 25 degrees C. It was also observed that combo cryogels could be repeatedly used for adsorption and desorption cycles of lysozyme molecules. The purification efficiency of adsorbent was highlighted as 83.4% with 88.2% purification yield from hen egg white via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).